[KO Validated] Anti-alpha Catenin Rabbit Polyclonal Antibody - P35221

Core Product Specifications and Parameters

Biological Background: Alpha-Catenin Function and Localization

• Alpha-catenin (CTNNA1) is a key component of cadherin-based adherens junctions, linking cadherins to the actin cytoskeleton and playing a critical role in cell-cell adhesion.
• It associates with both E- and N-cadherins, forming complexes that are essential for cadherin-mediated adhesive properties. Related references: PMID:40983751
• Homodimeric CTNNA1 can regulate actin filament assembly by competing with the Arp2/3 complex for actin filament binding, thereby inhibiting actin branching.
• CTNNA1 may play a crucial role in cell differentiation and is involved in the regulation of YAP1 and TGFB1-dependent SMAD2/3 nuclear accumulation.
• Subcellular localization includes the cytoplasm, cytoskeleton, adherens junctions, cell membrane, and nucleus, reflecting its dynamic role in cell adhesion and signaling.
• Ubiquitously expressed in normal tissues, with abundant expression in brain and cerebellum; also detected in placenta, liver, lung, colon, heart, pancreas, stomach, and thymus.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation (Ubl), which may modulate its function and stability.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 637-906 of human alpha-catenin, targeting a central region; consider this when designing domain-specific experiments.
• For Western blotting, a specific band at approximately 100 kDa is expected; validate with appropriate positive and negative controls, as the antibody is KO-validated.
• The antibody is suitable for IHC-P, IP, and ELISA; optimize dilutions and protocols empirically for each application and sample type.
• Cross-reactivity with mouse and rat orthologs has been demonstrated, making it suitable for model organism studies; however, validation in your specific experimental context is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw-material procurement, offering a comprehensive portfolio that includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For research on cell adhesion, adherens junctions, and cadherin biology, this alpha-catenin antibody exemplifies our commitment to providing rare and critical target reagents. We bridge the gap from concept to clinic, ensuring reliable supply and technical support for diagnostic manufacturers and research laboratories worldwide.