[KO Validated] Anti-Ajuba Rabbit Polyclonal Antibody for WB, ELISA - Q96IF1

Core Product Specifications and Parameters

Biological Background: Ajuba Function and Localization

• Ajuba is a LIM domain-containing scaffold protein encoded by the AJUBA gene, involved in the assembly of diverse protein complexes that govern cell fate, cytoskeletal organization, and gene transcription.
• It contributes to epithelial cell-cell junction integrity by linking cadherin-based adhesive receptors to the actin cytoskeleton, thereby strengthening cell adhesion.
• Ajuba acts as a transcriptional corepressor for multiple factors, including SNAI1 and SNAI2/SLUG, to suppress E-cadherin expression, and also serves as an HDAC-dependent corepressor for a subset of GFI1 target genes.
• The protein localizes to several subcellular compartments, including the cytoplasm, cytoskeleton, cell membrane, cell junctions, nucleus, centrosome, and P-bodies, reflecting its multifaceted roles.
• In mitotic regulation, Ajuba modulates AURKA kinase activity, contributing to mitotic commitment and progression.
• It functions in hypoxia signaling by bridging prolyl hydroxylases and VHL, thereby promoting efficient degradation of HIF1A under normoxic conditions.
• Ajuba negatively regulates the Hippo signaling pathway by antagonizing YAP1 phosphorylation, thus influencing organ size control and tumorigenesis.
• The protein contains LIM domains that mediate protein-protein interactions and zinc binding, and it undergoes alternative splicing and phosphorylation, which may modulate its diverse cellular activities.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region spanning amino acids 201–290, which lies within the central portion of Ajuba; this design may provide good specificity across isoforms.
• For Western blotting, a band near 57 kDa is expected; consider using reducing conditions and validate detection with appropriate controls (e.g., knockout cell lysates or siRNA-treated samples).
• In ELISA applications, coating with the recombinant immunogen or full-length protein can serve as a positive control; optimize antibody dilutions empirically for your assay system.
• When working across species, note the documented cross-reactivity with human and rat; verify performance in other relevant samples if needed.
• Given Ajuba’s involvement in multiple signaling pathways, consider pathway-specific stimuli (e.g., IL-1β, hypoxia) to assess context-dependent protein expression or localization changes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with integrated access to IVD raw materials. In the context of Ajuba-related studies—from cell adhesion and transcriptional regulation to cancer biology—we can supply validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our procurement network also specializes in sourcing rare-target raw materials, streamlining your development from concept to clinic.