[KO Validated] Anti-α-Catenin Monoclonal Antibody for WB - P35221

Core Product Specifications and Parameters

Biological Background: α-Catenin (Alpha E-catenin) Function and Localization

• Alpha-catenin (catenin alpha-1, Alpha E-catenin, CTNNA1) is a cadherin-associated protein that links cadherins to the actin cytoskeleton at adherens junctions, playing a critical role in cell-cell adhesion. Related references: PMID:40983751
• The homodimeric form of α-catenin regulates actin filament assembly and inhibits actin branching by competing with the Arp2/3 complex for binding to actin filaments.
• α-Catenin is involved in the regulation of WWTR1/TAZ, YAP1 and TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation, implicating it in cell differentiation.
• It localizes to the cytoplasm, cytoskeleton, adherens junctions, cell membrane, and nucleus.
• α-Catenin is ubiquitously expressed in normal tissues, with abundant expression in brain, cerebellum, placenta, liver, lung, colon, heart, pancreas, stomach, and thymus.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (Ubl conjugation), as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human α-Catenin; consider this epitope specificity when designing experiments.
• For Western blotting, the predicted protein weight is 100 kDa. Validate detection in relevant sample systems (human, mouse, rat) and use appropriate molecular weight markers.
• ELISA applications may benefit from pairing with a capture antibody targeting a different epitope; cross-reactivity with mouse and rat can facilitate multispecies studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this anti-α-Catenin monoclonal antibody for cell adhesion and junction research. Our supply capabilities include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support diagnostic development from concept to clinic. Contact us to explore how we can streamline your next project.