[KO Validated] AK2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P54819

Core Product Specifications and Parameters

Biological Background: Adenylate Kinase 2 (AK2) Function and Localization

• AK2 (adenylate kinase 2) is a mitochondrial enzyme that catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP, producing two molecules of ADP.
• This reaction is central to cellular energy homeostasis and adenine nucleotide metabolism, regulating phosphate utilization and AMP de novo biosynthesis pathways.
• AK2 plays a critical role in hematopoiesis, and its deficiency is associated with reticular dysgenesis, a severe form of severe combined immunodeficiency (SCID).
• The protein localizes to the mitochondrial intermembrane space, distinguishing it from the cytosolic adenylate kinase 1 (AK1).
• AK2 is widely expressed, with highest levels in heart, liver, and kidney, and lower levels in brain, skeletal muscle, and skin. It is present in thrombocytes but absent in erythrocytes, which lack mitochondria.
• In leukocytes, AK2 is the predominant adenylate kinase, as AK1 expression is low or absent, making these cells particularly dependent on AK2 for energy metabolism.
• The enzyme undergoes post-translational modifications including acetylation and phosphorylation, and contains conserved ATP-binding and kinase motifs characteristic of the adenylate kinase family.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human AK2 protein (amino acids 1–232), ensuring recognition of the native protein across multiple applications.
• For western blot (WB), the predicted molecular weight is approximately 26 kDa; consider using heart or liver tissue lysates as positive controls based on high AK2 expression in these organs.
• For immunohistochemistry (IHC-P), antigen retrieval may be necessary; validate staining in known AK2-rich tissues such as cardiac muscle or kidney.
• Reported cross-reactivity with mouse and rat suggests suitability for rodent model studies; however, verify epitope conservation by sequence alignment before use in these species.

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