[KO Validated] ACLY Rabbit Monoclonal Antibody for WB, IP, IF/ICC, ELISA - P53396

Core Product Specifications and Parameters

Biological Background: ACLY Function and Localization

• ATP-citrate synthase (ACLY) is a key metabolic enzyme that catalyzes the ATP-dependent cleavage of citrate into oxaloacetate and acetyl-CoA, linking carbohydrate and lipid metabolism.
• The produced acetyl-CoA is a crucial substrate for de novo lipid synthesis, protein acetylation, and other biosynthetic processes, positioning ACLY as a central regulator of cellular energy homeostasis.
• ACLY belongs to the citrate synthase family and is evolutionarily conserved, with the human protein encoded by the ACLY gene (GeneID: 47) and comprising multiple isoforms through alternative splicing.
• The protein localizes predominantly in the cytoplasm/cytosol, where it accesses mitochondrially derived citrate to support lipogenesis and histone acetylation in the nucleus after translocation.
• Post-translational modifications, including phosphorylation and acetylation, modulate ACLY enzymatic activity and stability, with phosphorylation at specific serine residues being responsive to insulin and nutrient signals.
• Keywords associated with ACLY include ATP-binding, magnesium-binding, phosphoprotein, and transferase activity, reflecting its catalytic mechanism and regulatory roles.
• Dysregulation of ACLY expression or activity has been implicated in metabolic disorders and cancer, making it a target of interest for therapeutic intervention.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1000–1101 of human ACLY (P53396). For optimal results in WB, consider denaturing gel electrophoresis and transfer conditions suitable for high-molecular-weight proteins (~121 kDa).
• For immunofluorescence (IF/ICC), include appropriate fixation and permeabilization steps to preserve cytoplasmic localization. Validate antibody specificity using knockout or knockdown controls where possible.
• In immunoprecipitation (IP) experiments, determine the optimal antibody-to-lysate ratio empirically and confirm co-immunoprecipitated proteins with relevant controls. The antibody’s KO validation supports its specificity in WB applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents tailored to metabolic and oncology research needs. As a one-stop sourcing partner, we provide convenient access to ACLY-targeting materials and can assist with rare target sourcing to support diagnostic development from concept to clinic. Contact us to streamline your procurement with reliable, research-grade reagents.