KMT2A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q03164

Core Product Specifications and Parameters

Biological Background: KMT2A Function and Localization

• KMT2A (MLL1, ALL-1, HRX) is the catalytic subunit of the MLL1/MLL complex, a histone methyltransferase that predominantly generates H3K4me1 and H3K4me2 marks at active chromatin regions, facilitating transcription and DNA repair. Related references: PMID:12453419 PMID:15960975 PMID:19187761
• The enzyme has low methyltransferase activity on its own and requires assembly with other core complex members (e.g., WDR5, ASH2L, RBBP5) to achieve full catalytic competence. Related references: PMID:19187761 PMID:26886794
• It binds unmethylated CpG islands in target gene promoters, maintaining their hypomethylated state, and is critical for the transcriptional activation of HOXA9, a key developmental regulator. Related references: PMID:12453419 PMID:20010842 PMID:20677832
• KMT2A localizes predominantly to the nucleus.
• It is expressed in heart, lung, brain, and T- and B-lymphocytes, consistent with its roles in development and hematopoiesis.
• Participates in the circadian clock by mediating rhythmic H3K4 methylation, which establishes a permissive chromatin state for CLOCK-BMAL1 binding (By similarity).
• Undergoes auto-methylation at Cys-3882, a modification that may modulate its enzymatic activity. Related references: PMID:24235145
• KMT2A is a proto-oncogene frequently involved in chromosomal rearrangements that produce oncogenic fusion proteins, driving acute lymphoblastic and myeloid leukemias.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 2829–2883 near the C‑terminus; consider that this polyclonal antibody may recognize full‑length KMT2A (~432 kDa) as well as certain isoforms or proteolytic fragments.
• For Western blot, use low‑percentage (e.g., 6%) SDS‑PAGE gels, extended wet transfer times, and include protease inhibitors in lysis buffer to preserve the large 432 kDa protein.
• In immunofluorescence/immunocytochemistry, expect nuclear staining; co‑stain with a nuclear marker (e.g., DAPI) and validate specificity in knock‑down or knockout controls.
• For ELISA, standard indirect or sandwich protocols can be employed; titrate the antibody to determine optimal working concentrations for your specific assay system.
• Cross‑reactivity with mouse and rat orthologs is reported; however, verify sequence homology and reactivity in the relevant species' lysates before large‑scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one‑stop partner for IVD raw materials, offering validated KMT2A antibodies and ancillary reagents for epigenetic and oncology research. From optimized polyclonal antibodies and matched detection pairs to bulk serum and recombinant proteins, we streamline your procurement for diagnostic development. Whether you need established catalog products or custom sourcing for rare targets, CamelBio bridges the gap from concept to clinic with reliable supply solutions.