KLK10 (NES1) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O43240

Core Product Specifications and Parameters

Biological Background: Kallikrein-10 Function and Localization

• Kallikrein-10 (KLK10), also designated normal epithelial cell-specific 1 (NES1) and protease serine-like 1 (PRSSL1), is a secreted serine protease belonging to the kallikrein-related peptidase family.
• It functions as a tumor suppressor, with documented roles in inhibiting breast and prostate cancer progression.
• The protein is secreted and contains a signal sequence, consistent with its extracellular role in proteolytic cascades.
• KLK10 is primarily expressed in hormone-responsive tissues, including breast, ovary, and prostate.
• The mature protein has a predicted molecular weight of approximately 30 kDa and features a conserved serine protease domain with disulfide bonds critical for structural stability.
• Post-translational modifications include N-linked glycosylation, which may influence its secretion and functional activity.
• UniProt annotations link KLK10 to processes including cell cycle regulation and proteolysis, and classify it as a secreted glycoprotein with tumor suppressor activity.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 43–276, which corresponds to the mature protease domain; thus, the antibody is likely to recognize the secreted, processed form of KLK10.
• For Western blotting, expect a band near 30 kDa under reducing conditions; validate molecular weight in your lysate type as glycosylation may affect migration.
• In immunofluorescence/immunocytochemistry, permeabilization may be needed to detect intracellular pools, although extracellular staining is possible for secreted protein.
• For ELISA applications, the antibody may serve as a capture or detection reagent; users should titrate and pair-test in their specific assay format.
• Cross-reactivity with mouse and rat KLK10 is reported; confirm sequence homology of epitope region if quantitative comparison is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and labs, offering validated antibody pairs, optimized polyclonal antibodies like this anti-KLK10 pAb, and bulk ancillary reagents. With expertise in tumor-suppressor targets and secreted cancer biomarkers, we support your assay development from concept to clinic. Contact us to discuss custom sourcing, technical consultations, and scalable supply solutions.