KLHL9 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9P2J3

Core Product Specifications and Parameters

Biological Background: KLHL9 Function and Localization

• KLHL9 (Kelch-like protein 9) functions as a substrate-specific adapter of the BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex.
• It is critically required for mitotic progression and the completion of cytokinesis.
• The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex specifically mediates the ubiquitination of Aurora kinase B (AURKB).
• Through AURKB regulation, KLHL9 controls the dynamic behavior of AURKB on mitotic chromosomes, ensuring faithful chromosome segregation.
• KLHL9 contains characteristic BTB and kelch repeat domains and is annotated with the Ubl conjugation pathway keyword, reflecting its role in ubiquitin-mediated processes.
• The protein is a component of the reference proteome and has been identified in high-throughput proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-100) of human KLHL9, which may be suitable for detecting the full-length protein across species.
• For Western blot, the predicted molecular weight is approximately 69 kDa; consider verifying band specificity using relevant controls or siRNA knockdown.
• In IHC-P, KMHL9 expression may be cell-cycle dependent; validate in synchronized or proliferating tissue models where mitosis is active.
• For ELISA, the polyclonal nature of the antibody can enhance detection sensitivity; titrate the antibody accordingly for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This KLHL9 antibody supports research into cell cycle regulation and mitosis, and we can assist with rare target sourcing, custom development, and technical consultation to accelerate your project from concept to clinic.