KLF6 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q99612

Core Product Specifications and Parameters

Biological Background: KLF6 Function and Localization

• Also known as Zf9, COPEB, CPBP, and BCD1, KLF6 is a zinc-finger transcription factor from the Krüppel-like factor family.
• Functions as a transcriptional activator that binds to GC-rich promoter elements, regulating genes involved in cell growth and differentiation.
• Contains three C-terminal C2H2-type zinc finger motifs responsible for DNA binding and transcriptional activity.
• Localizes to the nucleus where it interacts with target gene promoters.
• Widely expressed, with highest levels in placenta, spleen, thymus, prostate, testis, small intestine, and colon; lower expression in pancreas, lung, liver, heart, and skeletal muscle.
• Implicated in B-cell development and may act as a tumor suppressor in certain contexts.
• May undergo alternative splicing, producing isoforms with potentially distinct regulatory roles.
• Included in proteomics identification and serves as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 40–200 of human KLF6, the N-terminal region upstream of the zinc-finger domains; this sequence may be conserved in mouse, consistent with reported cross-reactivity.
• For Western blot, expect a band around 32 kDa; validate in positive control lysates such as placenta or spleen with appropriate molecular weight markers.
• In immunohistochemistry (paraffin-embedded sections), consider antigen retrieval optimization and validate in tissues known to express KLF6.
• For immunofluorescence or ICC, nuclear staining is expected; include DAPI counterstain to confirm localization.
• Titrate the antibody for each application and sample type using a dilution series to determine optimal working concentration.
• When using mouse models, perform parallel control experiments to confirm cross-reactivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers IVD manufacturers and research laboratories a single access point to validated antibody raw materials, including this KLF6 polyclonal antibody for transcription factor studies and cancer biology applications. Our supply portfolio encompasses optimized monoclonal and polyclonal antibodies, matched antibody pairs, bulk ancillary reagents, and tailored sourcing solutions for rare or low-abundance targets. Partner with us to streamline your raw material procurement from discovery through scale-up.