KLF17 Rabbit Polyclonal Antibody for WB and ELISA - Q5JT82

Core Product Specifications and Parameters

Biological Background: KLF17 Function and Localization

• KLF17 (Krueppel-like factor 17), also known as zinc finger protein 393 (ZNF393), is a member of the KLF family of transcription factors, characterized by three C2H2-type zinc fingers at its C-terminus.
• Functions primarily as a transcription repressor, binding to the specific DNA sequence 5'-CACCC-3' in the promoter regions of target genes to inhibit their expression.
• Acts as a negative regulator of epithelial-mesenchymal transition (EMT) and metastasis, particularly in breast cancer, by directly repressing the expression of ID1, a key metastasis regulator.
• In germ cells, KLF17 may serve as a germ cell-specific transcription factor involved in spermatid differentiation and oocyte development (by similarity).
• Subcellular localization is restricted to the nucleus, consistent with its role in transcriptional regulation.
• Classified as a metal-binding, zinc-finger protein with dual activator/repressor potential, as reflected by its UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 210-389 of human KLF17, which includes the C-terminal zinc finger domain region, suggesting that the antibody may recognize epitopes within these conserved DNA-binding motifs.
• For Western blot applications, expect a specific band at approximately 43 kDa in nuclear extracts of human or mouse cells; validate in a relevant sample system such as breast cancer cell lines.
• In ELISA, consider using recombinant KLF17 protein as coating antigen; optimize antibody dilution and detection conditions empirically.
• Since cross-reactivity with mouse is reported, the antibody may be suitable for studies in mouse xenograft or transgenic models; always include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw-material procurement, offering end-to-end support from concept to clinic. For KLF17-related research in transcriptional regulation and cancer biology, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing of rare targets to streamline your assay development and manufacturing needs.