KIF2B Rabbit pAb for WB, ELISA - Q8N4N8

Core Product Specifications and Parameters

Biological Background: KIF2B Function and Localization

• KIF2B is a member of the kinesin-13 family of microtubule-depolymerizing motor proteins, essential for microtubule dynamics during cell division.
• It acts as a plus end-directed microtubule-dependent motor that depolymerizes microtubules, a function critical for spindle assembly and chromosome movement (PubMed:17538014). Related references: PMID:17538014
• KIF2B specifically contributes to chromosome congression during mitosis, ensuring proper chromosome alignment at the metaphase plate (PubMed:23891108). Related references: PMID:23891108
• The protein localizes to the cytoplasm and associates with the microtubule cytoskeleton, including centrosomes, mitotic spindles, and kinetochores, reflecting its dynamic roles in mitosis.
• KIF2B is highly expressed in lung and ovary, with moderate levels in heart, kidney, placenta, skeletal muscle, and spleen. A shorter isoform is detected in pancreas and spleen.
• As a phosphoprotein with ATP- and nucleotide-binding domains, KIF2B is regulated by phosphorylation and operates in an ATP-dependent manner during the cell cycle.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (residues 544–673) of human KIF2B; this region may contribute to specific detection, but cross-reactivity with related kinesins should be assessed experimentally.
• For Western blot, a band near 76 kDa is expected; use positive control lysates from human, mouse, or rat tissues with high KIF2B expression (e.g., lung) and validate specificity with siRNA or knockout samples.
• In ELISA applications, the antibody may be suitable as a capture or detection reagent; optimization of coating conditions and detection system is recommended.
• Being a polyclonal antibody, it can recognize multiple epitopes, potentially increasing sensitivity but also the risk of non-specific binding; consider using appropriate blocking buffers and dilution optimizing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic with validated antibody pairs, bulk ancillary reagents, and rare target sourcing. This anti-KIF2B polyclonal antibody supports cell division and mitosis research, and we also offer complementary reagents for streamlined assay development. Explore our catalog to efficiently access high-quality antibodies and raw materials tailored to your diagnostic needs.