KIAA1456 Rabbit Polyclonal Antibody (TRM9L) for WB, ELISA - Q9P272

Core Product Specifications and Parameters

Biological Background: KIAA1456 (TRMT9B) Function and Localization

• KIAA1456 (also known as TRMT9B, TRM9L, hTRM9L, or C8orf79) is a probable tRNA methyltransferase 9B, a member of the methyltransferase superfamily.
• It plays a role in tRNA modification, potentially catalyzing the formation of wobble uridine modifications in specific arginine and glutamic acid tRNAs, thereby impacting translation fidelity.
• Functions as a tumor suppressor by upregulating the expression of LIN9, a key regulator of cell cycle progression.
• Exhibits down-regulated expression in multiple carcinomas, including breast, bladder, colorectal, cervix, and testicular cancers, suggesting its involvement in tumor suppression.
• The protein undergoes alternative splicing and is a phosphoprotein, indicating that it is subject to regulatory post-translational modifications.
• The theoretical molecular weight is approximately 51 kDa (51299 Da), consistent with the observed protein weight for this antibody.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein corresponding to amino acids 110-330 of human KIAA1456. This region may be conserved across human, mouse, and rat, supporting cross-reactivity. Validate expression in your cell line or tissue of interest.
• For Western blot, a band at ~51 kDa is expected. Due to potential post-translational modifications or alternative splicing, multiple bands or slight size shifts may be observed. Optimize sample preparation (e.g., reducing vs. non-reducing conditions) and use appropriate blocking to minimize background.
• For ELISA, titrate the antibody and verify optimal coating conditions. Include appropriate controls to account for polyclonal antibody variability.
• Reported to cross-react with human, mouse, and rat. Cross-reactivity with each species should be confirmed under your experimental conditions.
• As a rabbit polyclonal antibody, it may recognize multiple epitopes, offering robust detection but potentially higher non-specific binding; consider adjusting blocking agents and washing stringency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials, including this anti-KIAA1456 polyclonal antibody for cancer research applications. Our comprehensive portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to support your assay development. As a trusted sourcing partner, CamelBio also facilitates procurement of rare target raw materials, bridging the gap from concept to clinic.