KIAA0513 Rabbit Polyclonal Antibody for WB and ELISA - O60268

Core Product Specifications and Parameters

Biological Background: KIAA0513 Function and Localization

• KIAA0513 is an uncharacterized protein with alternative splicing, suggesting the existence of multiple isoforms.
• The protein is localized to the cytoplasm, as indicated by subcellular location annotations.
• KIAA0513 is widely expressed, with highest levels detected in brain regions including cerebellum, brain cortex, hippocampus, pons, putamen, and amygdala. It is highly expressed in neurons and also present in glial cells.
• Expression analysis shows slightly elevated protein levels in the dorsolateral prefrontal cortex of individuals with schizophrenia compared to controls, hinting at a potential role in neuropsychiatric conditions.
• The protein is classified as a phosphoprotein, implying regulation by phosphorylation-mediated signaling pathways.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant fusion protein spanning amino acids 1-301 of human KIAA0513, which is likely to detect multiple isoforms if the epitope region is conserved.
• For Western blot (WB), consider using protein extracts from brain tissue or cell lines known to express KIAA0513; a band around 47 kDa is expected, though isoforms may produce additional bands.
• For ELISA, it may be beneficial to use purified recombinant KIAA0513 protein or the immunogen fragment as a positive control to assess binding.
• Cross-reactivity with human and mouse has been indicated; validate reactivity in the relevant species and sample system before critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibodies like this anti-KIAA0513 polyclonal antibody to support research in neuroscience and uncharacterized protein biology. Our supply capabilities span optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw material sourcing, helping diagnostic developers move efficiently from concept to clinic.