[KD Validated] STAT2 Rabbit Monoclonal Antibody for WB, ELISA - P52630

Core Product Specifications and Parameters

Biological Background: STAT2 Function and Localization

• STAT2 (Signal transducer and activator of transcription 2), also known as p113, is a key mediator of type I interferon (IFN-alpha/beta) signaling. Upon receptor binding, TYK2 and JAK1 phosphorylate STAT2, allowing dimerization with STAT1 and association with IRF9 to form the ISGF3 complex, which activates interferon-stimulated genes (ISGs) via ISRE elements. Related references: PMID:23391734, PMID:9020188
• Beyond its transcriptional activator role, STAT2 also exerts negative feedback on type I IFN signaling by recruiting USP18 to the IFNAR2 receptor subunit, mitigating the antiviral response. Related references: PMID:28165510
• STAT2 is involved in regulating mitochondrial fission by modulating DNM1L phosphorylation at Ser-616 (activation) and Ser-637 (inactivation), linking innate immunity to mitochondrial dynamics. Related references: PMID:23391734, PMID:26122121
• The protein contains an SH2 domain and undergoes phosphorylation; it is a phosphoprotein and subject to ubiquitin-like conjugation (Ubl conjugation) as highlighted by UniProt keywords.
• STAT2 localizes to both the cytoplasm and the nucleus, with translocation occurring upon activation. Keywords include DNA-binding, transcription regulation, and antiviral defense.
• Alternative splicing of STAT2 generates multiple isoforms, and disease-associated variants have been reported (UniProt keyword: Disease variant).
• The protein interacts with host machinery during viral infection, indicated by the "Host-virus interaction" keyword; it is also identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 550–706 of human STAT2 (NP_005410.1). Verify that your target isoform or experimental conditions preserve this epitope region.
• For Western blot, anticipate a band near 98 kDa. Consider using interferon-alpha-stimulated cell lysates as positive controls to enhance detection.
• For ELISA, the antibody may be used for capture or detection; validate pairing and cross-reactivity with related STAT family proteins in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials. Our portfolio includes validated monoclonal antibodies like this STAT2 antibody, optimized for WB and ELISA. We also offer antibody pairs, ancillary reagents, and custom sourcing for rare targets, supporting your entire workflow from concept to clinic.