[KD Validated] Rabbit Anti-TIMM13 Monoclonal Antibody for WB, IF/ICC, ELISA - Q9Y5L4

Core Product Specifications and Parameters

Biological Background: TIMM13 Function and Localization

• TIMM13 encodes Mitochondrial import inner membrane translocase subunit Tim13, a ~10.5 kDa mitochondrial intermembrane space chaperone (UniProt: Q9Y5L4).
• It acts as a chaperone-like protein that prevents aggregation of hydrophobic precursors and guides them through the mitochondrial intermembrane space during import.
• Together with TIMM8, it forms the TIMM8-TIMM13 complex, which specifically mediates import of multi-pass inner membrane proteins such as TIMM23, SLC25A12/ARALAR1, and SLC25A13/ARALAR2; the related TIMM9-TIMM10 complex handles a wider array of substrates.
• Localized to the mitochondrial inner membrane, consistent with its role in the later stages of protein translocation.
• Ubiquitously expressed, with highest levels in heart, kidney, liver, and skeletal muscle—tissues with high mitochondrial content and energy demand.
• The protein undergoes post-translational modifications including acetylation and phosphorylation, and contains conserved cysteine residues that form disulfide bonds; it also binds zinc ions, characteristic of small Tim chaperones.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 95 amino acids of human TIMM13, suggesting the antibody recognizes the full-length native protein; consider using recombinant TIMM13 protein or a positive control lysate (e.g., HeLa or HEK293) for initial validation.
• For Western blotting, the predicted molecular weight is approximately 11 kDa; use a 15% SDS-PAGE gel and optimize transfer conditions for small proteins to ensure sharp bands.
• In immunofluorescence/ICC, mitochondrial staining is expected; confirm specificity by co-staining with a mitochondrial marker such as Tom20 or using MitoTracker.
• The antibody is validated for ELISA; consider pairing with a capture antibody for sandwich ELISA applications targeting TIMM13, and validate specificity with recombinant antigen.
• As cross-reactivity is confirmed only for human, test in human-derived samples and be mindful of potential species-specific epitope variations when designing experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, offering validated monoclonal antibodies like this anti-TIMM13 clone to support research in mitochondrial biology and protein transport disorders. From optimized antibody pairs for sandwich ELISA to bulk ancillary reagents, CamelBio’s supply chain accelerates assay development and production. For rare or emerging targets, our sourcing team can locate custom raw materials to meet your project’s unique needs.