[KD Validated] Pin1 Rabbit mAb for WB and ELISA - Q13526

Core Product Specifications and Parameters

Biological Background: Pin1 (Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) Function and Localization

• Pin1 is a peptidyl-prolyl cis-trans isomerase (PPIase) that binds and isomerizes phosphorylated Ser/Thr-Pro motifs, functioning as a molecular switch in diverse cellular processes. Related references: PMID:21497122 PMID:22033920 PMID:23623683 PMID:29686383
• Regulates mitosis by interacting with NIMA and attenuating its activity; down-regulates kinase activity of BTK. Related references: PMID:16644721
• Required for efficient dephosphorylation and recycling of RAF1 after mitogen activation. Related references: PMID:15664191
• Binds and targets PML and BCL6 for proteasomal degradation in a phosphorylation-dependent manner. Related references: PMID:17828269
• Regulates JNK signaling by binding phosphorylated FBXW7, disrupting its dimerization and promoting its autoubiquitination and degradation, leading to JUN stabilization. Related references: PMID:22608923
• Localizes to the nucleus, nuclear speckles, and cytoplasm.
• The phosphorylated form at Ser-71 is expressed in normal breast tissue cells but not in breast cancer cells.
• Acts as a negative regulator of adipocyte browning by binding and promoting degradation of phosphorylated PRDM16 (By similarity).

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1-163 of human Pin1, covering the full-length protein; consider using this antibody for detecting endogenous Pin1 in human, mouse, and rat samples.
• For Western blot, a band around 18 kDa is expected. Validate specificity using Pin1 knockdown (KD) samples or peptide competition.
• For ELISA, optimize coating conditions and pair with a suitable detection antibody; cross-reactivity with Pin1 from mouse and rat allows multi-species assays.
• Since Pin1 is phosphorylated at Ser-71 in normal tissues, confirm detection in relevant sample systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing bridge for IVD raw materials, supplying validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-Pin1 rabbit monoclonal antibody supports cell cycle and signaling research, and we can assist with rare target raw-material sourcing to accelerate your assay development from concept to clinic.