[KD Validated] MRPL11 Rabbit pAb - Q9Y3B7

Core Product Specifications and Parameters

Biological Background: Large Ribosomal Subunit Protein uL11m Function and Localization

• MRPL11 encodes the mitochondrial large ribosomal subunit protein uL11m, also known as L11mt or MRP-L11, a key component of the 39S ribosomal subunit.
• The protein is essential for mitochondrial protein synthesis, facilitating the translation of 13 mtDNA-encoded polypeptides critical for oxidative phosphorylation.
• Alternative splicing of the MRPL11 gene produces multiple transcript variants, potentially giving rise to distinct protein isoforms with tissue-specific or developmental roles.
• MRPL11 localizes exclusively to the mitochondrial matrix, consistent with its role in mitochondrial ribosomes and the presence of an N-terminal mitochondrial transit peptide.
• As a ribosomal protein, uL11m participates in ribosome assembly, tRNA binding, and peptidyl transferase activity within the mitoribosome.
• Structural studies have contributed to understanding the unique features of the human mitoribosome, including the incorporation of MRPL11 at the GTPase-associated center.
• Proteomics identification has confirmed MRPL11 expression across multiple human tissues, validating its ubiquitous presence in mitochondria.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 91-192) of human MRPL11, a sequence conserved across isoforms; confirm identity in your specific sample system when using alternative splicing variants.
• For Western blotting, consider running mitochondrial-enriched lysates to enhance detection, as MRPL11 is a low-abundance mitochondrial protein.
• The antibody is suitable for immunoprecipitation; optimize the antibody-to-lysate ratio and include appropriate negative controls to minimize nonspecific binding.
• When using knockdown validation, verify MRPL11 depletion at both mRNA and protein levels, as residual ribosomal pools may confound interpretation.
• ELISA applications may require pairing with a capture or detection antibody; titrate both components to achieve optimal signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to supply high-quality IVD raw materials, including validated antibodies like this anti-MRPL11 polyclonal antibody, suitable for mitochondrial research and respiratory chain disorder studies. Our portfolio spans optimized monoclonal/polyclonal antibodies, recombinant proteins, and ancillary reagents, supported by technical expertise to accelerate your assay development from concept to clinic. For bulk orders or custom sourcing of rare-target antibodies, contact CamelBio to bridge your procurement needs efficiently.