[KD Validated] MCT1 Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - P53985

Core Product Specifications and Parameters

Biological Background: MCT1 Function and Localization

• MCT1 (SLC16A1) is a proton-coupled monocarboxylate transporter that mediates the bidirectional movement of lactate, pyruvate, acetate, and ketone bodies across the plasma membrane, helping maintain intracellular pH and contributing to metabolic homeostasis. Related references: PMID:12946269 PMID:33333023
• MCT1 serves as a major lactate exporter, playing a critical role in cellular responses to high-fat diet by modulating lactate and pyruvate levels, which in turn influence central metabolic pathways and insulin secretion.
• The transporter facilitates the export of protonated succinate during muscle cell acidification in exercising muscle and ischemic heart, a process important for metabolic regulation. Related references: PMID:32946811
• MCT1 function relies on alternate outward- and inward-open conformational states, with protonation and deprotonation of Asp309 being essential for this transition. Related references: PMID:33333023
• MCT1 localizes to the cell membrane, with expression reported on both basolateral and apical surfaces in polarized cells.
• MCT1 is widely expressed in tissues, with detection in heart, blood lymphocytes, and monocytes at the protein level. Related references: PMID:12115955 PMID:15505343 PMID:15901598
• The protein is subject to alternative splicing and phosphorylation, and has been associated with disease variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 190–245 of human MCT1, a region likely exposed in native and denatured conditions. Consider this antibody for applications such as WB, IF, and IHC-P, and validate performance in the relevant sample type.
• KD validation indicates suitability for knockdown experiments; confirm efficacy in your cellular model.
• For ELISA, evaluate direct or sandwich formats with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material sourcing, from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents. For MCT1-related research in metabolism and cancer, we offer rapid access to rare target materials and technical support spanning concept to clinic.