[KD Validated] GPX4 Rabbit pAb for WB, IF-P, ELISA - P36969

Core Product Specifications and Parameters

Biological Background: GPX4 Function and Localization

• GPX4 (glutathione peroxidase 4) is a selenocysteine-containing enzyme that directly reduces phospholipid hydroperoxide, protecting cells from membrane lipid peroxidation. Related references: PMID:40281343

• It plays an essential anti-ferroptotic role by preventing the iron-dependent accumulation of lipid reactive oxygen species. Related references: PMID:24439385 PMID:40281343

• In platelets, GPX4 participates in arachidonic acid metabolism by reducing hydroperoxy ester lipids from the 15-lipoxygenase pathway. Related references: PMID:11115402

• GPX4 can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide, and tert-butyl hydroperoxide. Related references: PMID:17630701 PMID:36608588

• It is required for normal sperm development, male fertility, and maturation/survival of photoreceptor cells, as demonstrated in model organisms.

• The protein is present in mitochondria and the cytoplasm, with testis and platelets expressing the highest levels in humans.

• Multiple isoforms exist due to alternative initiation, and post-translational modifications include selenocysteine at the active site, hydroxylation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen (aa 30–197 of human GPX4) targets a conserved core region; this polyclonal antibody may recognize multiple GPX4 isoforms and cross-react with mouse/rat.
• For Western blot, analyze samples under reducing conditions and validate the ~22 kDa band in the relevant cell or tissue lysate.
• Consider testing immunofluorescence-paraffin (IF-P) on fixed sections after antigen retrieval optimization.
• Given the selenocysteine-dependent activity, ensure that the detection system is compatible with the redox status of the sample.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with high-quality IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies. This GPX4 antibody supports oxidative stress and ferroptosis-related assay development, and we offer bulk supply, customized immunoreagents, and rare-target sourcing to advance your projects from concept to clinic.