[KD Validated] FOXK1 Rabbit mAb - P85037

Core Product Specifications and Parameters

Biological Background: FOXK1 Function and Localization

• Forkhead box protein K1 (FOXK1), also known as myocyte nuclear factor (MNF), is a transcriptional regulator with a predicted molecular weight of ~75 kDa.
• Transcriptional regulator that binds the forkhead DNA sequence motif (5'-GTAAACA-3') and can act as either an activator or repressor depending on the context. Related references: PMID:17670796
• Key regulator of aerobic glycolysis: together with FOXK2, promotes expression of glycolytic enzymes (HK2, PFK, PKLR, LDH) and suppresses mitochondrial pyruvate oxidation via PDK1/4 upregulation.
• Negative regulator of autophagy in skeletal muscle: in response to starvation, represses autophagy genes to limit proteolysis.
• Regulates myogenic progenitor cells: promotes proliferation by repressing FOXO4 and inhibits differentiation by blocking MEFC activity.
• Positively modulates Wnt/β-catenin signaling by mediating DVL translocation into the nucleus. Related references: PMID:25805136
• Antiviral function: reduces virus replication by binding the interferon stimulated response element (ISRE) to induce antiviral gene expression. Related references: PMID:25852164
• Acts as an accessory component of the polycomb repressive deubiquitinase (PR-DUB) complex, recruiting it to specific target genes. Related references: PMID:24634419 PMID:30664650
• Indirect positive regulator of ferroptosis: upon PRKCB phosphorylation, FOXK1 promotes LGALS13 expression and secretion. Related references: PMID:40246981
• Subcellular localization: primarily nuclear, but also detected in the cytoplasm.
• Tissue expression: found in developing and adult tissues, with notable expression in brain, colon, and lymph node tumors.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 600-733); the antibody may detect all FOXK1 isoforms.
• For Western blot, a band at approximately 75 kDa is expected in human and mouse samples; use appropriate loading controls.
• Validated for multiple applications: WB, IP, IF/ICC, IHC-P, ChIP, ELISA; consider optimizing conditions for each assay, particularly antigen retrieval for IHC and sonication/cross-linking for ChIP.
• Cross-reacts with human and mouse; verify reactivity if using other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This FOXK1 antibody supports research in metabolic regulation, muscle biology, and cancer, and CamelBio can facilitate procurement of additional rare targets and customized production to accelerate your diagnostic development.