KD Validated DNMT1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P26358

Core Product Specifications and Parameters

Biological Background: DNMT1 Function and Localization

• DNA (cytosine-5)-methyltransferase 1 (DNMT1), also known as CXXC9, MCMT, AIM, HSN1E, ADCADN, and m.HsaI, is essential for maintaining DNA methylation patterns through cell division.
• Methylates CpG residues, with a preference for hemimethylated DNA, ensuring epigenetic inheritance during DNA replication.
  Related references:
  PMID:17200670
  PMID:18754681
  PMID:21745816
  PMID:26070743
• Associates with DNA replication sites in S phase to copy the methylation pattern to the newly synthesized strand, and also binds chromatin during G2/M for replication-independent maintenance.
  Related references:
  PMID:17200670
  PMID:21745816
• Mediates transcriptional repression by directly interacting with histone deacetylase 2 (HDAC2).
  Related references:
  PMID:10888872
• Cooperates with DNMT3B and CTCFL/BORIS to activate BAG1 gene expression through modulation of histone H3 dimethylation (H3K4 and H3K9).
  Related references:
  PMID:18413740
• Forms a corepressor complex required for KRAS-mediated promoter hypermethylation and silencing of tumor suppressor genes in colorectal cancer cells; also maintains transcriptional repression in undifferentiated embryonic stem cells.
  Related references:
  PMID:24623306
• Localizes to the nucleus and chromosomes. Ubiquitously expressed, with highest levels in fetal tissues, heart, kidney, placenta, and peripheral blood mononuclear cells.
• Contains a CXXC-type zinc finger domain; subject to post-translational modifications including acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-124 of DNMT1, an N-terminal region that may be exposed in both full-length and some truncated isoforms. For applications like western blotting (WB), use reducing conditions and appropriate molecular weight markers around 183 kDa.
• Due to the large size of DNMT1 (~183 kDa), consider using low-percentage polyacrylamide gels (e.g., 6-8%) and extended transfer times to ensure efficient blotting.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and permeabilization conditions for your specific tissue or cell type.
• The antibody is reported to cross-react with human, mouse, and rat samples. Validate specificity in your experimental system, using appropriate positive and negative controls.
• For ELISA, the antibody can be used as a detection or capture reagent; pairing with other DNMT1 antibodies may require testing for optimal performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials for epigenetics research, including high-quality antibodies against DNA methylation targets such as DNMT1. Whether you need validated polyclonal antibodies, recombinant proteins, or bulk ancillary reagents, CamelBio streamlines your sourcing from concept to clinic. Leverage our expertise in diagnostic development and rare target sourcing to accelerate your projects with reliable, ready-to-use biomaterials.