[KD Validated] COPB2 Rabbit PolymAb® - P35606

Core Product Specifications and Parameters

Biological Background: COPB2 Function and Localization

• COPB2 encodes the beta'-COP subunit of the coatomer complex, a 102 kDa protein also known as p102 or beta'-coat protein.
• The coatomer complex is a cytosolic hetero-oligomer that binds to dilysine motifs on cargo proteins and associates with Golgi-derived COPI-coated vesicles, mediating retrograde transport from the Golgi to the endoplasmic reticulum (ER).
• COPB2 is essential for Golgi membrane budding and contributes to Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
• The protein localizes to the cytoplasm, cytosol, Golgi apparatus membrane, and COPI-coated vesicle membranes.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomic studies.
• Alternative splicing produces multiple isoforms; mutations in COPB2 are associated with primary microcephaly (MCPH19) and osteoporosis.
• The protein contains multiple WD repeat domains that mediate protein-protein interactions within the coatomer and with cargo.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 657–906 of human COPB2, a C-terminal region; the antibody may detect full-length COPB2 and potentially certain isoforms.
• For western blot (WB), the expected band is approximately 102 kDa; validate the band pattern in relevant human or mouse cell/tissue lysates, and consider using a knockdown or knockout control if available.
• In immunofluorescence (IF/ICC), expect a Golgi and vesicular staining pattern; co‑stain with a Golgi marker (e.g., GM130) to confirm subcellular localization.
• For immunohistochemistry (IHC‑P), optimize antigen retrieval conditions and use appropriate controls; COPB2 expression levels vary among tissues.
• The antibody is listed as suitable for ELISA; consider titration and pairing with a detection antibody if used in sandwich ELISA formats.
• General recommendation: Include positive and negative experimental controls, and empirically determine the optimal working dilution for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, supporting manufacturers and researchers from concept to clinic. For COPB2 and Golgi transport studies, we offer validated antibody pairs, optimized monoclonal antibodies like this COPB2 Rabbit PolymAb®, and bulk ancillary reagents. Our team can also assist in sourcing rare target reagents, ensuring seamless integration into your diagnostic assay development pipeline.