[KD Validated] CDX2 Rabbit pAb - Q99626

Core Product Specifications and Parameters

Biological Background: CDX2 Function and Localization

• CDX2, also known as CDX-3, is a homeobox transcription factor encoded by the CDX2 (CDX3) gene, belonging to the caudal-related homeobox gene family.
• It acts as a master regulator of intestinal epithelial differentiation and maintenance, controlling genes critical for the absorptive and secretory functions of intestinal cells.
• CDX2 binds to the promoters of various intestine-specific genes, including sucrase-isomaltase (SI), lactase-phlorizin hydrolase (LPH), vitamin D receptor (VDR), claudin-2 (CLDN2), and mucin-2 (MUC2), to activate their transcription.
• It exhibits a preference for binding to methylated DNA, which may influence gene regulation in the intestinal epithelium. Related references: PMID:28473536
• Subcellularly, CDX2 localizes to the nucleus, consistent with its role as a transcription factor.
• Post-translational modifications include phosphorylation and ubiquitin conjugation, as indicated by proteomics data.
• CDX2 expression is tissue-specific, detected primarily in the small intestine, colon, and pancreas.
• It plays a crucial role in embryonic development and is implicated in maintaining intestinal identity and suppressing tumorigenesis in colorectal cancers.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 14-165) of human CDX2, which is highly conserved among human, mouse, and rat, supporting cross-reactivity.
• For Western blotting, anticipate a band around 34 kDa; use appropriate positive controls such as intestinal tissue lysates or colorectal cancer cell lines (e.g., Caco-2, HT-29).
• In immunofluorescence/ICC, nuclear staining is expected; consider validating with a nuclear counterstain.
• This polyclonal antibody may be suitable for ELISA; titration is recommended for optimal signal-to-noise ratio.
• As the antibody is validated by KD (knockdown), confirm specificity in your system by using siRNA or CRISPR-Cas9 mediated CDX2 knockdown samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing solution for diagnostic manufacturers and researchers, offering CDX2 and other intestinal transcription factor antibodies alongside a wide portfolio of IVD raw materials. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents to support your assay development from concept to clinic. Our team specializes in sourcing rare targets, ensuring you have reliable access to the critical components needed for your gastrointestinal disease diagnostics and research.