[KD Validated] CDK2 Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - P24941

Core Product Specifications and Parameters

Biological Background: CDK2 Function and Localization

• CDK2 (Cyclin-dependent kinase 2, p33) is a serine/threonine-protein kinase essential for cell cycle progression, particularly the G1-S transition and S phase entry, where it phosphorylates RB1 to release E2F1 and promote transcription.
  Related references: PMID:10499802, PMID:10995386

• It triggers centrosome duplication by phosphorylating NPM1, and drives the S-to-G2 progression through cyclin A/CDK2-mediated activation of histone gene transcription via NPAT phosphorylation.
  Related references: PMID:11051553, PMID:10995387

• CDK2 phosphorylates BRCA2 to regulate homologous recombination-dependent DNA repair, with low phosphorylation during active recombination in S phase and increased phosphorylation toward mitosis; it also phosphorylates NBN for telomere repair.
  Related references: PMID:15800615, PMID:20195506, PMID:28216226

• The kinase localizes to multiple subcellular compartments, including the nucleus (Cajal bodies), cytoplasm, centrosomes, and endosomes, consistent with its pleiotropic functions.

• CDK2 activity is controlled by cyclin E/A binding, activating phosphorylation by the CDK7/cyclin H/MAT1 CAK complex, and S-nitrosylation; it also serves as a substrate for USP37 deubiquitinase at the G1-S transition.
  Related references: PMID:41100585, PMID:20079829, PMID:21596315

• In embryonic stem cells, CDK2 orchestrates a balance between proliferation, apoptosis, and DNA repair, and its inactivation mediates vitamin D-induced growth inhibition.
  Related references: PMID:18372919, PMID:19238148, PMID:20147522

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the C-terminal region (amino acids 200-298) of human CDK2. This region is conserved in mouse and rat, supporting cross-reactivity, but validate in your relevant sample system.
• For Western blot, endogenous CDK2 is typically detected around 30–34 kDa, with slower-migrating bands representing phosphorylated forms. Consider using phosphatase inhibitors in lysis buffers.
• Suitable for immunofluorescence (IF/ICC and IF-P) and immunohistochemistry (IHC-P). Optimization of fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) conditions is recommended.
• For ELISA, pairing with a suitable capture antibody and validating with recombinant CDK2 protein is advised.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio provides diagnostic manufacturers and research labs with seamless access to high-quality antibodies and ancillary reagents. This CDK2 rabbit monoclonal antibody supports cell cycle and cancer research applications, and we offer validated antibody pairs, bulk quantities, and custom sourcing for rare targets to accelerate your assay development. From concept to clinic, CamelBio is your trusted sourcing bridge for complete IVD solutions.