[KD Validated] Anti-ZNF143 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, and ELISA - P52747

Core Product Specifications and Parameters

Biological Background: ZNF143 Function and Localization

• ZNF143 is a transcription factor that activates expression of genes transcribed by both RNA polymerases II and III, and is required to safeguard mitochondrial activity.
  Related references: PMID:10893243, PMID:14702349, PMID:17938208

• Specifically recognizes and binds the Staf-binding site (SBS), a consensus DNA-binding motif present in thousands of promoters.
  Related references: PMID:17092945, PMID:21177654, PMID:23408857

• Activates the gene for selenocysteine tRNA (tRNAsec).
  Related references: PMID:23152058

• Activates expression of small nuclear RNA (snRNA) transcribed by RNA polymerases II and III.
  Related references: PMID:17938208, PMID:9776743

• Acts as a key regulator of cell proliferation and differentiation by specifically activating expression of a subset of nuclear-encoded mitochondrial genes, thereby controlling mitochondrial function (By similarity).

• Involved in the maintenance of embryonic stem cells by promoting association of POU5F1/OCT4 to promoters, leading to NANOG expression (By similarity).

• Subcellular localization: Nucleus, Chromosome.

• Contains zinc-finger domains and is subject to post-translational modifications including acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 592-638 of human ZNF143, a region near the C-terminus. This rabbit monoclonal antibody may recognize full-length ZNF143.
• Suitable for Western blot (predicted band ~69kDa) and has been knock-down validated; consider using it to assess ZNF143 knockdown efficiency in your model system.
• For IHC-P and IF/ICC, nuclear staining is expected; appropriate antigen retrieval and fixation protocols may enhance detection.
• ELISA can be employed for quantitative detection; optimize coating conditions and antibody titration for best results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. For mitochondrial transcription factor research, including ZNF143, we offer validated monoclonal antibodies, antibody pairs, and bulk ancillary reagents to streamline your assay development. Our expertise in rare target procurement ensures fast access to essential components for your workflow.