KD Validated Anti-YTHDC2 Rabbit Polyclonal Antibody for WB, IP, IF-P, ELISA - Q9H6S0

Core Product Specifications and Parameters

Biological Background: YTHDC2 Function and Localization

• YTHDC2 is a 3'-5' RNA helicase that plays a key role in germline development by promoting the transition from mitotic to meiotic divisions; it is essential for spermatogenesis and oogenesis. Related references: PMID:26318451 PMID:29033321 PMID:29970596
• It specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification affecting mRNA processing and stability. Related references: PMID:26318451 PMID:29033321
• YTHDC2 promotes degradation of m6A-containing mRNAs through its 3'-5' RNA helicase activity; this degradation may involve the XRN1 exoribonuclease. Related references: PMID:29033321
• YTHDC2 belongs to the YTH domain-containing protein family and contains ANK repeats, a helicase ATP-binding domain, and a YTH RNA-binding domain.
• Subcellular localization is predominantly cytoplasmic, with enrichment in the perinuclear region.
• Testis-specific expression is observed, with strong upregulation in spermatocytes upon entry into meiosis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 1286–1430) of YTHDC2, which lies distal to conserved helicase and YTH domains; this may reduce cross-reactivity with other helicase family members in WB and IP applications.
• For Western blotting, a band at ~160 kDa is expected; consider using a high molecular weight marker and validate in relevant testis or germ cell lines.
• In immunofluorescence (IF-P), permeabilization and fixation conditions may require optimization due to the cytoplasmic and perinuclear localization of YTHDC2.
• Cross-reactivity with mouse and rat has been reported; validate species-specific signals with appropriate controls.
• For ELISA applications, antigen coating conditions may affect antibody binding; titrate antibody concentration for optimal sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our YTHDC2 antibody supports research in germline development and RNA epigenetics. For your assay development needs, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and sourcing assistance for rare targets.