[KD Validated] Anti-YTHDC1 Monoclonal Antibody for WB, IHC-P, IP, ELISA - Q96MU7

Core Product Specifications and Parameters

Biological Background: YTHDC1 Function and Localization

• YTH domain-containing protein 1 (YTHDC1), also known as YT521-B or splicing factor YT521, is a member of the YTH domain family of RNA-binding proteins that specifically recognizes N6-methyladenosine (m6A)-modified RNAs. Related references: PMID:25242552, PMID:26318451, PMID:26876937

• YTHDC1 functions as a key regulator of alternative splicing, where it binds m6A-containing pre-mRNAs and recruits splicing factors. Interaction with SRSF3 promotes exon inclusion, while competition with SRSF10 leads to inhibition of exon skipping. Related references: PMID:26876937

• It also plays a role in nuclear export of m6A-modified mRNAs through simultaneous binding to SRSF3 and the nuclear export factor NXF1, facilitating mRNA trafficking from the nucleus to the cytoplasm. Related references: PMID:28984244

• YTHDC1 is involved in the regulation of X-chromosome inactivation by recognizing m6A on Xist RNA and promoting its transcriptional repression activity, thereby contributing to the silencing of one X chromosome in female mammals. Related references: PMID:27602518

• Beyond RNA, YTHDC1 can also bind m6A-containing single-stranded DNA, suggesting broader roles in nucleic acid-mediated processes. Related references: PMID:32663306

• Subcellularly, YTHDC1 localizes predominantly to the nucleus and is enriched in nuclear speckles, which are storage and modification sites for splicing factors, consistent with its role in pre-mRNA processing.

• Post-translational modifications include phosphorylation and ubiquitination (isopeptide bond formation), which may regulate its activity and stability. The protein has a predicted molecular weight of 84.7 kDa, with an observed band at approximately 85 kDa by western blotting.

• YTHDC1 is essential for germline development, with studies in model organisms showing its requirement for spermatogonial development in males and oocyte growth in females, likely through its alternative splicing functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal 71 amino acids of human YTHDC1. Because the epitope maps to a region shared by multiple splice variants, the antibody may detect several isoforms; validation in the relevant cell line or tissue is recommended.
• Western blot analysis using this antibody is expected to show a band near 85 kDa. Lysates from human, mouse, or rat cells can be used, but the precise band pattern should be confirmed under the experimental conditions employed.
• For immunohistochemistry (IHC‑P), a predominantly nuclear signal, often concentrated in nuclear speckles, is anticipated. Optimization of antigen retrieval and antibody dilution may be required for each tissue type.
• The antibody is suitable for immunoprecipitation (IP) of endogenous YTHDC1; co‑immunoprecipitation of interacting partners such as SRSF3 can be explored with appropriate controls.
• ELISA applications can be developed using this antibody as a capture or detection reagent; pairing with a polyclonal detector or a matched antibody pair is advisable for sandwich ELISA formats.
• The knockdown (KD) validation confirms that the signal is reduced upon YTHDC1 depletion, supporting its specificity for target identification in gene‑silencing studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw‑material partner, offering validated antibodies like this YTHDC1 rabbit monoclonal antibody to support research into m6A modification, splicing regulation, and epigenetics. With a portfolio spanning monoclonal and polyclonal antibodies, antibody pairs, and ancillary reagents, we help diagnostic manufacturers and laboratories accelerate assay development from concept to clinic. For custom requirements, including bulk production and target-specific sourcing, our technical team provides tailored solutions to meet your project needs.