[KD Validated] Anti-XPNPEP3 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9NQH7

Core Product Specifications and Parameters

Biological Background: XPNPEP3 Function and Localization

• Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Leu-Pro-Ala; also shows low activity towards peptides with Ala or Ser at the P1 position. Related references: PMID:25609706 PMID:28476889
• Promotes TNFRSF1B-mediated phosphorylation of MAPK8/JNK1 and MAPK9/JNK2, acting as an adapter protein independent of its catalytic activity, and may inhibit TNF-TNFRSF1B-induced apoptosis.
• Two isoforms exist: isoform 1 is more abundant and widely expressed compared to isoform 2.
• Subcellular localization is primarily mitochondrial, but also cytoplasmic.
• Requires manganese as a cofactor and contains an N-terminal transit peptide for mitochondrial import.
• Mutations in XPNPEP3 cause nephronophthisis, an autosomal recessive cystic kidney disease.
• The molecular weight of the mature protein is approximately 57 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 32–230 of human XPNPEP3, comprising the catalytic domain; the antibody may recognize both isoforms and potentially inhibit enzymatic activity.
• For western blot, the predicted molecular weight is ~57 kDa. Consider using mitochondrial-enriched fractions or whole cell lysates from kidney or other relevant tissues, and include appropriate controls for knockdown validation.
• For IHC-P, antigen retrieval conditions may need optimization; validate in tissue sections such as kidney or other tissues with known XPNPEP3 expression.
• Cross-reactivity with mouse and rat enables studies in rodent models; confirm specificity and performance in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. With expertise spanning from concept to clinic, CamelBio supports your XPNPEP3-related projects—whether targeting nephronophthisis biomarkers or investigating TNF signaling pathways—by sourcing even rare-target materials efficiently.