KD Validated Anti-Vimentin Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P08670

Core Product Specifications and Parameters

Biological Background: Vimentin Function and Localization

• Vimentin is a class-III intermediate filament protein encoded by the VIM gene, with alternative names such as CTRCT30 and HEL113.
• It is highly expressed in mesenchymal cells and fibroblasts, with lower levels in T- and B-lymphocytes, and is often used as a marker for epithelial-mesenchymal transition (EMT).
• Vimentin plays a structural role by linking the nucleus, endoplasmic reticulum, and mitochondria to the cytoskeleton, facilitating cell shape, organelle positioning, and directional migration.
• It is involved in Golgi complex polarization at the cell migration front and may promote axon outgrowth and motor fiber repair through recruitment to outgrowth tips.
• The protein collaborates with LARP6 to stabilize type I collagen mRNAs (CO1A1 and CO1A2), linking it to extracellular matrix regulation.
• Subcellularly, vimentin localizes to the cytoplasm, cytoskeleton, nuclear matrix, cell membrane, and cell projections such as axons.
• Post-translational modifications include acetylation, phosphorylation, S-nitrosylation, and ubiquitylation, which regulate filament dynamics, solubility, and interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-101) of human vimentin; this antibody may detect full-length and N-terminal fragments — consider verifying reactivity with truncated or modified forms in your specific assay.
• For Western blot (WB), prepare reduced denatured samples and use appropriate molecular weight markers; a band around 54 kDa is expected for full-length vimentin.
• In immunohistochemistry (IHC-P), antigen retrieval methods such as heat-induced epitope retrieval (HIER) with citrate buffer (pH 6.0) or EDTA (pH 9.0) may enhance detection; include detergent permeabilization for IF/ICC.
• This antibody shows cross-reactivity with mouse and rat vimentin, so it can be used in multi-species studies; however, validate in the relevant tissue or cell line to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to high-quality IVD raw materials for cytoskeletal and cancer biomarker development. As a trusted sourcing bridge, we supply validated antibody pairs, optimized polyclonal antibodies like this anti-vimentin product, and bulk ancillary reagents to accelerate your assay development from concept to clinic. Contact us for customized sourcing of rare targets and one-stop technical support.