[KD Validated] Anti-TRADD Monoclonal Antibody for WB, IP, ELISA - Q15628

Core Product Specifications and Parameters

Biological Background: TRADD Function and Localization

• TRADD is an adapter protein that links TNFRSF1A/TNFR1 to FADD, mediating the signaling cascade leading to apoptosis and NF-κB activation.
  Related references: PMID:23955153, PMID:7758105, PMID:8612133
• Overexpression of TRADD itself is sufficient to induce both apoptosis and NF-κB pathways, underscoring its central role in TNF-induced cellular responses.
  Related references: PMID:7758105, PMID:8612133
• The nuclear isoform of TRADD acts as a tumor suppressor by binding TRIP12 and preventing ubiquitination-dependent degradation of the p19ARF tumor suppressor.
• TRADD localizes to the nucleus, cytoplasm, and cytoskeleton, reflecting its multifunctional roles in signaling and structural interactions.
• TRADD is ubiquitously expressed in all human tissues, consistent with its fundamental function in innate immune and cell death pathways.
• As a glycoprotein subject to alternative splicing, TRADD may exist in multiple post-translationally modified forms that influence its function and localization.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of TRADD is approximately 34 kDa; use a suitable loading control and validate the antibody with a known TRADD-positive cell line such as HeLa or Jurkat.
• When performing immunoprecipitation, confirm that the antibody can efficiently pull down endogenous TRADD from the lysate of relevant species (human, mouse, rat) under non-denaturing conditions.
• ELISA applications may require optimization of coating antigen and detection conditions; consider using recombinant TRADD protein as a standard for quantitative assays.
• Although cross-reactivity with mouse and rat has been demonstrated, verify specificity in the experimental system of interest using knockout or knockdown controls if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering customized antibodies, antigen reagents, and technical support from R&D to manufacturing. This TRADD antibody supports research into TNF signaling, apoptosis, and cancer biology—fields that benefit from our validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents. Whether you require antibody optimization, large-scale production, or sourcing of rare targets, CamelBio accelerates your development pipeline with reliable, high-quality supplies.