[KD Validated] Anti-Thymidylate synthase Monoclonal Antibody for WB, IHC-P, ELISA - P04818

Core Product Specifications and Parameters

Biological Background: Thymidylate synthase Function and Localization

• Thymidylate synthase (TYMS) is a key methyltransferase that catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), essential for DNA synthesis and repair.
• This enzyme plays a central role in the de novo nucleotide biosynthesis pathway, contributing to both nuclear and mitochondrial thymidylate pools.
• Alternative splicing generates multiple isoforms, which may fine‑tune enzyme activity or subcellular targeting.
• TYMS localizes to the nucleus, cytoplasm, and mitochondria, including the mitochondrial matrix and inner membrane, supporting compartment‑specific dTMP production.
• Post‑translational modifications include phosphorylation, isopeptide bond formation, and ubiquitin‑like conjugation, which may regulate enzyme stability and activity.
• Mutations in TYMS are associated with dyskeratosis congenita, a rare multi‑system disorder, highlighting its importance in rapidly dividing tissues.

Experimental Guidance and Technical Tips

• The antibody is raised against the full‑length human TS protein (aa 1‑313), making it suitable for detecting native TS in human samples.
• In WB, expect a band at approximately 36 kDa; validate the signal using knockdown or knockout controls as this product has been KD‑validated.
• For IHC‑P, consider standard antigen retrieval protocols and validate staining with appropriate tissue controls.
• Since cross‑reactivity is reported only for human, verify reactivity if used on other species.
• As a rabbit monoclonal antibody, it offers consistent lot‑to‑lot performance for reproducible assays.

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