KD Validated Anti-TFEB Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P19484

Core Product Specifications and Parameters

Biological Background: TFEB Function and Localization

• Transcription factor EB is a master regulator of lysosomal biogenesis, autophagy, and related metabolic and immune processes.
  Related references: PMID:21617040, PMID:22343943, PMID:22576015
• It binds E-box and CLEAR-box DNA motifs upon dimerization with MiT/TFE family members like TFE3 or MITF.
  Related references: PMID:1748288, PMID:19556463, PMID:29146937, PMID:22692423
• Nutrient-sensitive MTOR phosphorylation controls TFEB subcellular localization: phosphorylation retains it in the cytosol, while dephosphorylation drives nuclear import.
  Related references: PMID:21617040, PMID:22343943, PMID:22576015, PMID:22692423
• TFEB promotes autophagy-related gene expression, positively regulating the autophagic pathway.
  Related references: PMID:21617040, PMID:22576015, PMID:23434374
• During bacterial infection, itaconate alkylates TFEB, triggering nuclear translocation and lysosomal gene activation to aid pathogen clearance.
  Related references: PMID:35662396
• TFEB localizes to the nucleus, cytoplasm, and lysosome membrane, reflecting its multifaceted roles in cellular homeostasis.
• It is subject to phosphorylation, alternative splicing, and ubiquitination, and plays roles in adaptive immunity.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 50–173) covers the N-terminal region common to human TFEB isoforms, likely generating antibodies that recognize both full-length and potential splice variants. Consider validating band patterns in relevant lysates.
• For WB, the predicted molecular weight is 53 kDa, but phosphorylation may cause a slight upward shift. Use nuclear/cytoplasmic fractionation or TFEB knockout/knockdown samples to confirm specificity.
• In IF/ICC, TFEB exhibits cytoplasmic retention under nutrient-replete conditions and nuclear accumulation upon starvation or lysosomal inhibition. Include appropriate controls to verify subcellular localization changes.
• For IHC-P, antigen retrieval and signal amplification may be necessary; test with known positive tissues such as kidney or liver, where TFEB expression is documented.
• When using ELISA, the antibody can be employed as a capture or detection reagent paired with recombinant TFEB protein; optimize coating concentrations and blocking conditions.
• Cross-reactivity with mouse and rat enables comparative studies, but always validate in the species-specific biological context due to potential epitope variations.
• As a polyclonal antibody, lot-to-lot performance can vary; routine quality control using a reference standard is recommended to ensure reproducible results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material sourcing, bridging diagnostic manufacturers and research laboratories with high-quality antibodies for targets such as transcription factor EB. This polyclonal antibody supports investigations into autophagy, lysosomal disorders, and metabolic signaling pathways. Beyond individual reagents, CamelBio can supply validated antibody pairs, bulk ancillary materials, and assist in procuring rare-target raw materials, streamlining your transition from concept to clinical application.