KD Validated Anti-TFEB Rabbit Monoclonal Antibody for WB, IHC-P, ELISA, ChIP - P19484

Core Product Specifications and Parameters

Biological Background: TFEB Function and Localization

• TFEB (Transcription factor EB), also known as Class E basic helix-loop-helix protein 35 (bHLHe35), belongs to the MiT/TFE subfamily of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors, which includes MITF and TFE3.
• It functions as a master regulator of lysosomal biogenesis and autophagy by binding to coordinated lysosomal expression and regulation (CLEAR) motifs (5′-GTCACGTGAC-3′) in the promoters of lysosomal and autophagic genes. Related references: PMID:19556463, PMID:22692423
• TFEB activity is tightly controlled by nutrient-sensitive phosphorylation: under fed conditions, mTOR-mediated phosphorylation at multiple serine residues retains TFEB in the cytosol; starvation or lysosomal stress inhibits mTOR, leading to rapid dephosphorylation and nuclear translocation. Related references: PMID:21617040, PMID:22343943, PMID:22576015
• In the nucleus, TFEB dimerizes with itself or other MiT/TFE members to activate transcription of target genes involved in autophagy, lipid metabolism, energy homeostasis, and immune defense. Related references: PMID:1748288, PMID:29146937
• Additionally, TFEB plays a role in adaptive immunity by regulating CD40L expression in T-cells and in antibacterial innate immunity through itaconate-mediated alkylation that promotes its nuclear localization and lysosomal biogenesis. Related references: PMID:35662396
• Subcellular localization is dynamic: primarily cytoplasmic when phosphorylated, it translocates to the nucleus upon activation; it can also associate with the lysosomal membrane.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50–173 of human TFEB (NP_009093.1). This region lies within the N-terminal transcriptional activation domain, which is relatively conserved across human, mouse, and rat, supporting the reported cross-reactivity. For species not listed, sequence alignment is recommended.
• For Western blot, TFEB may appear as a doublet between 44 and 53 kDa due to differential phosphorylation or alternative splicing; using fresh phosphatase inhibitors in lysis buffer can help preserve phospho-isoforms. The antibody has been validated for knockdown specificity (KD Validated).
• In immunohistochemistry (IHC-P), antigen retrieval conditions should be optimized for your tissue type. Nuclear and cytoplasmic staining may be observed depending on the activation state of TFEB.
• For ChIP, ensure crosslinking and shearing conditions are optimized for your cell type; the antibody should efficiently enrich promoter regions containing CLEAR or E‑box sequences.
• Perform appropriate positive and negative controls (e.g., TFEB‑knockout lysates, known target gene loci) when establishing assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw-material procurement, supporting the entire development workflow from early stage research to clinical application. This TFEB antibody, targeting a key node in autophagy and lysosomal biology, is backed by our comprehensive offering of validated antibody pairs, monoclonal/polyclonal antibodies, bulk ancillary reagents, and bespoke sourcing for niche targets. Contact us to learn how we can accelerate your diagnostic assay development with reliable, high-quality raw materials.