KD Validated Anti-SULT2B1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O00204

Core Product Specifications and Parameters

Biological Background: SULT2B1 Function and Localization

• SULT2B1 belogns to the cytosolic sulfotransferase family 2B, with alternative names alcohol sulfotransferase and hydroxysteroid sulfotransferase 2 (gene SULT2B1, aliases HSST2, ARCI14).
• Functions as a sulfotransferase using 3'-phospho-5'-adenylyl sulfate (PAPS) as donor to catalyze sulfate conjugation. Responsible for sulfation of cholesterol and 3β-hydroxysteroids such as pregnenolone and DHEA (PubMed:12145317, PubMed:19589875, PubMed:16855051, PubMed:21855633). Related references: PMID:12145317 PMID:19589875 PMID:16855051 PMID:21855633
• Plays a critical role in epidermal cholesterol metabolism, regulating keratinocyte proliferation and differentiation (PubMed:28575648). Related references: PMID:28575648
• Alternative splicing produces isoforms with distinct substrate preferences: one isoform sulfonates cholesterol, while another sulfonates pregnenolone but not cholesterol.
• Localizes predominantly to the cytoplasm (cytosol), and also detected in microsomes and the nucleus, indicating dynamic intracellular distribution.
• Expressed in skin (stratum granulosum–stratum corneum junction), placenta, prostate, trachea, and at lower levels in small intestine and lung.
• Post‑translationally modified by phosphorylation; alternative splicing yields functionally distinct variants.
• Disease‑associated variant linked to ichthyosis (ARCI14), underscoring its clinical relevance in skin disorders.

Experimental Guidance and Technical Tips

• The immunogen comprises the full‑length human SULT2B1 sequence (amino acids 1–365), suggesting the antibody likely recognizes the native protein.
• For Western blot, the predicted molecular weight is 41 kDa; additional bands corresponding to splice variants may be observed. Validate in known positive tissues (e.g., placenta, skin) and consider peptide blocking or knockdown controls to confirm specificity.
• In IHC‑P applications, optimize antigen retrieval and include appropriate controls; staining should be consistent with reported cytoplasmic and nuclear localization.
• For ELISA, pair with a suitable matched antibody or use directly for antigen capture; always verify performance with recombinant protein or endogenous samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, from validated antibody pairs and monoclonal antibodies to bulk ancillary reagents. This anti‑SULT2B1 antibody is an excellent tool for metabolism and dermatology research, supporting assay development in areas such as steroid sulfation pathways and skin biology. Partner with CamelBio to streamline your sourcing needs and accelerate your diagnostic pipeline with reliable, high‑quality reagents.