[KD Validated] Anti-SREBF1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9WTN3

Core Product Specifications and Parameters

Biological Background: SREBF1 Function and Localization

• SREBF1 is synthesized as a precursor embedded in the endoplasmic reticulum membrane. Under low sterol conditions, it is cleaved to release a transcription factor that enters the nucleus and activates genes for cholesterol biosynthesis and lipid homeostasis (PubMed:11782483, PubMed:12855691, PubMed:19244231, PubMed:16100574). Related references: PMID:11782483 PMID:12855691 PMID:19244231 PMID:16100574
• The active SREBF1 binds SRE-1 and E-box motifs, regulating promoters of cholesterol biosynthesis genes and the LDL receptor pathway (PubMed:17290224, PubMed:19244231, PubMed:21459323). Binding to E-box motifs is inferred by similarity. Related references: PMID:17290224 PMID:19244231 PMID:21459323
• Isoform SREBP-1A is expressed in select tissues, has high transcriptional activity, and stimulates both lipogenic and cholesterogenic genes; it also contributes to innate immunity in macrophages (PubMed:8833906, PubMed:12855691, PubMed:21531336, PubMed:9062341). Related references: PMID:8833906 PMID:12855691 PMID:21531336 PMID:9062341
• Isoform SREBP-1C is the predominant form in most tissues, has weaker activity, and mainly controls lipogenic genes, strongly driving lipid synthesis in proliferating cells (PubMed:8833906, PubMed:9062341, PubMed:12855691, PubMed:21531336). Related references: PMID:8833906 PMID:9062341 PMID:12855691 PMID:21531336
• Subcellular localization: SREBF1 cycles between the endoplasmic reticulum membrane, Golgi apparatus membrane, COPII-coated vesicles, and nucleus in a sterol-dependent manner.
• Post-translational modifications include phosphorylation and ubiquitination, which regulate protein stability and transcriptional activity.
• Tissue specificity: SREBF1 is widely expressed. SREBP-1C predominates in liver, adrenal gland, brain, and adipose tissue, while SREBP-1A is selectively expressed in tissues such as intestinal epithelia and macrophages.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (amino acids 1-100) of mouse SREBP1. This region is conserved between mouse and human, supporting cross-reactivity.
• For Western blot, the full-length SREBF1 migrates at ~120 kDa; consider using appropriate gel percentages and transfer conditions for high-molecular-weight proteins. Sterol depletion may be needed to detect the processed nuclear form.
• In immunohistochemistry (IHC-P), validate antibody specificity using positive and negative control tissues; both human and mouse tissues can be tested due to cross-reactivity.
• For ELISA, use the immunizing peptide as a positive control and optimize coating conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. For studies on lipid metabolism and cholesterol biosynthesis, we offer optimized antibodies targeting key regulators like SREBF1, along with bulk supply and technical support to accelerate assay development. Contact us to discuss your specific sourcing needs.