[KD Validated] Anti-SOD1 Rabbit PolymAb® for WB, IP, IF, IHC, ELISA - P00441

Core Product Specifications and Parameters

Biological Background: SOD1 Function and Localization

• Superoxide dismutase [Cu-Zn] (SOD1), also known as hydrogen sulfide oxidase or hSod1, is a key antioxidant enzyme that destroys superoxide radicals produced within cells, protecting biological systems from oxidative toxicity. Related references: PMID:18948262 PMID:24140062
• It catalyzes the oxidation of hydrogen sulfide (H2S) to sulfate, thereby detoxifying H2S and limiting the accumulation of reactive sulfur species (RSS) including persulfides and polysulfides. Related references: PMID:36630448
• Subcellular localization: SOD1 is predominantly cytoplasmic but also detected in the nucleus.
• Mutations in SOD1 are associated with amyotrophic lateral sclerosis (ALS) and other neurodegenerative diseases, underscoring its vital role in neuronal health.
• Post-translational modifications include acetylation, phosphorylation, palmitoylation, and ubiquitin-like conjugation; SOD1 also forms a disulfide bond and coordinates copper and zinc metal ions, which are essential for its enzymatic activity.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant protein corresponding to amino acids 2-154 of human SOD1, covering nearly the full length of the mature protein. This design may contribute to recognition of the native protein in various applications.
• For Western blot (WB), a band at approximately 16 kDa is typically expected; pre-validate the antibody in your sample system, especially when comparing across human, mouse, and rat lysates, as sequence homology is high.
• When performing immunohistochemistry on paraffin-embedded sections (IHC-P), consider standard antigen retrieval methods and optimize antibody dilution using known positive control tissues (e.g., liver or kidney).
• For immunoprecipitation (IP) and immunofluorescence (IF), confirm that the antibody performs under your specific fixation and lysis conditions; recombinant rabbit monoclonal antibodies often provide consistent batch-to-batch reproducibility.
• ELISA application may require pairing with a suitable detection antibody; validate the assay sensitivity and specificity in your matrix of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for diagnostic developers and researchers, offering reliable access to validated IVD raw materials such as this anti-SOD1 antibody. Whether you need bulk monoclonal/polyclonal antibodies, optimized antibody pairs, or ancillary reagents for oxidative stress and neurodegenerative disease studies, our one-stop platform streamlines sourcing from concept to clinic. Let us help you secure critical components for your next diagnostic or research project.