KD Validated Anti-Sharpin Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q9H0F6

Core Product Specifications and Parameters

Biological Background: Sharpin Function and Localization

• Sharpin (Shank-associated RH domain-interacting protein), also known as SIPL1 (Shank-interacting protein-like 1), is a component of the linear ubiquitin chain assembly complex (LUBAC). LUBAC conjugates linear polyubiquitin chains in a head-to-tail manner to substrates, playing a key role in NF-kappa-B activation and inflammation regulation.
  Related references:
  PMID:21455173 PMID:21455180 PMID:21455181
• Through LUBAC, Sharpin mediates linear ubiquitination of IKBKG (NEMO) and RIPK1, which is essential for canonical NF-kappa-B and JNK signaling pathways. This modification interferes with TNF-induced cell death, thereby preventing inflammation.
  Related references:
  PMID:21455173 PMID:21455180 PMID:21455181
• In innate immunity, Sharpin-containing LUBAC conjugates linear polyubiquitin chains on the surface of cytosol-invading bacteria, forming a ubiquitin coat that serves as an 'eat-me' signal for xenophagy and promotes NF-kappa-B activation. LUBAC extends pre-existing ubiquitin rather than initiating ubiquitination.
  Related references:
  PMID:28481331
• Sharpin also participates in the regulation of canonical Wnt signaling during angiogenesis, together with the deubiquitinase OTULIN, by modulating linear ubiquitination events.
  Related references:
  PMID:23708998
• The protein localizes predominantly to the cytoplasm and cytosol, and is also found at synapses, consistent with its roles in signal transduction and neuronal function.
• Expression is highest in skeletal muscle and placenta, with lower levels in brain, heart, colon, thymus, spleen, kidney, liver, small intestine, lung, and peripheral blood leukocytes. Sharpin is upregulated in various tumor tissues, including kidney, liver, ovary, and pancreas.
• Sharpin contains zinc-finger domains and is a phosphoprotein, indicating regulation by post-translational modifications. It is subject to alternative splicing, generating multiple isoforms.

Experimental Guidance and Technical Tips

• The antibody was raised against an N-terminal fragment (aa 1-126) of human Sharpin. This region may be conserved across known isoforms; consider verifying isoform coverage if applicable.
• For Western blot (WB), a band of approximately 40 kDa is expected. Use positive control lysates such as HeLa or HEK293, which express Sharpin.
• In immunofluorescence/immunocytochemistry (IF/ICC), expect mainly cytoplasmic and possibly synaptic staining patterns. Optimize fixation and permeabilization for the cell type of interest.
• ELISA application is possible; perform appropriate antibody titration and use recombinant Sharpin protein as standard.
• As Sharpin is involved in TNF signaling, stimulation with TNF-alpha may affect its localization or complex formation, which could be probed using this antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw material procurement, offering one-stop access to validated antibodies, antigen proteins, and ancillary reagents. This KD validated Sharpin antibody supports NF-kappa-B and inflammation research, and we can further supply bulk quantities, matched pairs, and customized raw-material solutions to accelerate your diagnostic development from concept to clinic.