[KD Validated] Anti-SERPINA3 Rabbit Polyclonal Antibody for WB, ELISA - P01011

Core Product Specifications and Parameters

Biological Background: SERPINA3 Function and Localization

• Encoded by the SERPINA3 gene, alpha-1-antichymotrypsin belongs to the serine protease inhibitor (serpin) superfamily and is also known as ACT, AACT, GIG24, or GIG25.
• It inhibits neutrophil cathepsin G and mast cell chymase, which are capable of converting angiotensin-1 to the active vasopressor angiotensin-2.
• The protein is secreted and shows increased plasma levels during the acute phase of inflammation or infection, consistent with its synthesis in the liver.
• SERPINA3 has been identified in amyloid plaques from the hippocampus of Alzheimer disease brains, indicating potential involvement in neurodegenerative processes.
• UniProt annotation highlights features such as alternative splicing, glycosylation, and a signal peptide, underscoring post-translational processing and secretion.

Experimental Guidance and Technical Tips

• For Western blotting, consider using whole cell or tissue lysates from human liver or plasma samples, where SERPINA3 is abundant; a band around 48 kDa is expected.
• Validate antibody specificity by including positive and negative controls; recombinant SERPINA3 protein can serve as a positive control.
• For ELISA applications, optimize coating antigen concentration and antibody dilution empirically; the polyclonal nature may provide robust signal in sandwich or indirect formats.
• Since this antibody is raised in rabbit, use an appropriate anti-rabbit secondary antibody conjugated to HRP or alkaline phosphatase for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, from validated antibody pairs and bulk polyclonal antibodies to ancillary reagents. This anti-SERPINA3 antibody supports research into acute phase responses and neurodegenerative disease, and falls within our portfolio of protease inhibitor-related products. For custom requirements or rare target sourcing, our technical team is ready to assist with tailored supply solutions.