KD Validated Anti-RHOT1/MIRO1 Rabbit Polyclonal Antibody - Q8IXI2

Core Product Specifications and Parameters

Biological Background: RHOT1/MIRO1 Function and Localization

• RHOT1 (also known as MIRO1, ARHT1) is an atypical mitochondrial nucleoside-triphosphatase (NTPase) and a member of the Rho GTPase family.
• It is critically involved in mitochondrial trafficking and anterograde transport, regulating the subcellular distribution of mitochondria. Related references: PMID:12482879, PMID:16630562, PMID:22396657
• Under high calcium conditions, RHOT1 promotes mitochondrial fission, a process essential for mitochondrial dynamics. Related references: PMID:27716788
• The protein can hydrolyze GTP, ATP, and UTP, demonstrating broad nucleotide specificity. Related references: PMID:30513825
• RHOT1 localizes to the mitochondrial outer membrane via a C-terminal transmembrane domain.
• It is ubiquitously expressed, with highest levels in heart and skeletal muscle, reflecting its role in tissues with high metabolic demand.
• The UniProt record highlights additional features such as acetylation, alternative splicing, calcium regulation, GTP-binding, and isopeptide bond formation.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment spanning amino acids 310–590 of human RHOT1, which may provide robust recognition of the endogenous protein in multiple applications.
• For Western blot, expect a band at approximately 71 kDa; the protein weight may vary slightly due to post-translational modifications.
• The antibody is indicated for cross-reactivity with human, mouse, and rat samples, supporting its use in common model organisms; validation in the specific experimental system is advised.
• As a polyclonal antibody, consider batch-to-batch consistency and include positive controls (e.g., RHOT1-knockdown or -overexpression lysates) to confirm specificity.
• The KD validation suggests suitability for knockdown studies; confirm efficiency with appropriate siRNA or CRISPR controls.
• For immunofluorescence (IF/ICC), consider mitochondrial-specific co-stains to verify localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop sourcing solution for IVD raw materials, including well-characterized antibodies such as this anti-RHOT1 polyclonal, ideal for mitochondrial biology and trafficking studies. Our portfolio extends to validated antibody pairs, bulk ancillary reagents, and rapid access to rare target reagents—streamlining your project from concept to clinic. Whether you need routine supplies or customized molecular tools, CamelBio bridges your research and production requirements with B2B-oriented support and dependable supply chains.