[KD Validated] Anti-RARG Polyclonal Antibody for WB and ELISA - P13631

Core Product Specifications and Parameters

Biological Background: Retinoic Acid Receptor Gamma (RARG) Function and Localization

• RARG (retinoic acid receptor gamma) is a member of the nuclear receptor superfamily that functions as a ligand-dependent transcription factor.
• It binds all-trans and 9-cis retinoic acid and forms heterodimers with retinoid X receptors (RXRs) to regulate gene expression via retinoic acid response elements (RAREs), characterized by tandem AGGTCA motifs (DR1-DR5).
• In the absence of ligand, RARG acts predominantly as a transcriptional activator due to weak association with corepressors.
• RARG is critical for limb bud development and, in concert with RARA and RARB, is required for skeletal growth, matrix homeostasis, and growth plate function.
• Localization is primarily nuclear, but it can also be detected in the cytoplasm.
• At the protein level, RARG is expressed in aortic endothelial cells.
• The protein contains zinc-finger DNA-binding domains and is subject to post-translational modifications including methylation, phosphorylation, and ubiquitination.
• Alternative splicing leads to multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-70) of human RARG. For Western blot, consider starting with a standard dilution range and optimizing based on signal-to-noise ratio in your sample.
• This antibody is KD validated, making it suitable for knockdown confirmation experiments in human cell lines; include appropriate positive and negative controls.
• Reported applications are WB and ELISA; performance in other applications (e.g., IF, IHC) has not been determined and would require independent validation.
• Cross-reactivity has been confirmed for human; if using in other species, verify sequence homology and perform a pilot experiment.

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CamelBio serves as a comprehensive sourcing platform for IVD raw materials, connecting diagnostic manufacturers and researchers with high-quality reagents like this RARG antibody. In the field of nuclear receptor signaling and transcriptional regulation, CamelBio offers validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary bulk reagents to streamline your assay development. For rare or custom targets, our team provides specialized sourcing support to help bridge your procurement needs from concept to clinic.