KD Validated Anti-RAB27A Rabbit Monoclonal Antibody for WB, IF, IHC, ELISA - P51159

Core Product Specifications and Parameters

Biological Background: RAB27A Function and Localization

• RAB27A is a small GTPase of the Rab family that cycles between inactive GDP-bound and active GTP-bound forms, recruiting effectors to regulate vesicle formation, movement, tethering, and fusion. Related references: PMID:30771381
• It regulates homeostasis of the late endocytic pathway, controlling endosomal positioning, maturation, and secretion. Related references: PMID:30771381
• RAB27A is essential for cytotoxic granule exocytosis in lymphocytes, required for granule maturation and docking at the immunologic synapse. Related references: PMID:18812475
• Subcellularly, RAB27A associates with membranes of melanosomes, late endosomes, and lysosomes.
• It is widely expressed in most tissues except brain, with low levels in thymus, kidney, muscle, and placenta; detected in melanocytes, tumor cells, CTLs, and mast cells.
• Post-translational modifications include prenylation (lipoprotein), phosphorylation, acetylation, and methylation, critical for its function.
• The protein exists in alternatively spliced isoforms and is linked to human disease variants (e.g., Griscelli syndrome).
• UniProt lists a predicted molecular weight of 24868 Da, consistent with the ~25 kDa band observed in assays.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length RAB27A (aa 1-221), which is highly conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot, a band at approximately 25 kDa is expected; consider using positive controls such as lymphocytes, melanoma cell lines, or tissues rich in immune cells.
• For immunofluorescence (IF/ICC, IF-P), membrane/perinuclear staining consistent with endosomal/lysosomal localization is anticipated; validate with appropriate organelle markers.
• In ELISA applications, the recombinant protein immunogen can serve as a positive control.
• For IHC-P, test on tissues with known RAB27A expression, such as spleen or tumor tissues, and include tissue-specific controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like this anti-RAB27A clone, ideal for studying vesicle trafficking and immune cell exocytosis. We offer bulk quantities, custom antibody development, paired detection reagents, and rapid sourcing for rare targets to accelerate your assay development from concept to clinic.