KD Validated Anti-POLR2L Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P62875

Core Product Specifications and Parameters

Biological Background: POLR2L Function and Localization

• POLR2L encodes the RPABC5 subunit, a shared component of RNA polymerases I, II, and III, also known as RPB10, RPB7.6, or RBP10.
• It is a DNA-directed RNA polymerase that catalyzes the transcription of DNA into RNA using ribonucleoside triphosphates.
• As a common subunit, it participates in the synthesis of ribosomal RNA precursors (Pol I), mRNA and non-coding RNA (Pol II), and small RNAs like 5S rRNA and tRNAs (Pol III).
• The protein localizes to the nucleus, with specific enrichment in the nucleolus.
• POLR2L is a zinc-binding protein and a metal-binding transcription factor.
• Keywords: 3D-structure, DNA-directed RNA polymerase, Metal-binding, Nucleus, Proteomics identification, Reference proteome, Transcription, Zinc.

Experimental Guidance and Technical Tips

• Since the immunogen is a synthetic peptide, consider validating antibody specificity by peptide blocking assays.
• For Western blotting, the expected band is around 8 kDa; use an appropriate low-molecular-weight marker and membrane (e.g., PVDF with small pore size) to retain the protein.
• For IHC-P, ensure proper antigen retrieval conditions (heat-induced or enzymatic) are optimized for nuclear proteins.
• In ELISA, confirm optimal coating concentration and blocking buffer for the peptide antigen.

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