[KD Validated] Anti-PHF11 Rabbit Monoclonal Antibody for WB, ELISA - Q9UIL8

Core Product Specifications and Parameters

Biological Background: PHF11 Function and Localization

• PHF11 (PHD finger protein 11) is also known as BRCA1 C-terminus-associated protein (BCAP) and renal carcinoma antigen NY-REN-34. It contains a PHD-type zinc finger domain, characteristic of chromatin-associated proteins.
• PHF11 functions as a positive regulator of Th1-type cytokine gene expression, playing a role in adaptive immune responses.
• The protein is localized to the nucleus, consistent with its role in transcriptional regulation.
• PHF11 is highly expressed in immune cells, including T cells, B cells, natural killer cells, and mature dendritic cells. It shows higher expression in Th1 cells compared to Th2 cells.
• Low-level expression is observed in various normal tissues such as lung, testis, small intestine, breast, liver, and placenta.
• The protein is subject to alternative splicing, generating multiple isoforms. It is a metal-binding protein, coordinating zinc ions via its PHD finger.
• PHF11 is classified as a transcription regulator and activator, involved in transcription regulation.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fragment corresponding to amino acids 40–321 of human PHF11. Consider using this immunogen information for designing positive controls or blocking experiments.
• For Western blotting, the predicted molecular weight of human PHF11 is approximately 38 kDa (observed band may vary due to post-translational modifications or isoforms). Validate with appropriate positive controls from relevant cell lines or tissues.
• This antibody has been shown to cross-react with mouse and rat PHF11 by the supplier; however, users should verify reactivity in their specific experimental system.
• ELISA performance may require optimization of coating and detection conditions; include a standard curve and appropriate buffer controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing integrated access to antibodies, reagents, and technical support from discovery to commercialization. This anti-PHF11 monoclonal antibody targets a key regulator of Th1 immune responses, making it valuable for immunological and diagnostic research. Our supply capabilities include validated antibody pairs, optimized rabbit monoclonal antibodies, bulk ancillary reagents, and sourcing of rare targets to meet your production needs.