KD Validated Anti-PDXK Polyclonal Antibody for WB, IF/ICC, ELISA - O00764

Core Product Specifications and Parameters

Biological Background: Pyridoxal Kinase Function and Localization

• PDXK encodes pyridoxal kinase, also known as pyridoxine kinase, which belongs to the pyridoxal kinase family.
• It catalyzes the phosphorylation of vitamin B6 vitamers (pyridoxal, pyridoxine, pyridoxamine) to their active 5'-phosphate forms, with pyridoxal 5'-phosphate (PLP) serving as a cofactor for over 140 enzymes. Related references: PMID:10987144 PMID:17766369 PMID:19351586
• The protein is primarily localized in the cytoplasm and cytosol.
• PDXK is ubiquitously expressed, with particularly high levels in testis and spermatozoa. Related references: PMID:31187503 PMID:9099727
• It binds ATP and metal ions such as cobalt, magnesium, manganese, sodium, and zinc as cofactors for its kinase activity.
• The protein undergoes acetylation and phosphorylation, and alternative splicing produces multiple isoforms.
• Mutations in PDXK are associated with Charcot-Marie-Tooth disease and other neurodegenerative conditions, highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 213-312 of human PDXK; this region is predicted to be exposed and antigenic. Consider using this antibody for WB detection in whole cell lysates, as PDXK is cytoplasmic.
• For IF/ICC, fixation and permeabilization conditions should be optimized due to the cytoplasmic localization; validate in the relevant cell line.
• The antibody is reported to cross-react with mouse and rat; however, sequence alignment between species should be checked for the immunogen region to confirm specificity.
• As a polyclonal antibody, lot-to-lot variability may occur; consider including appropriate controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For vitamin B6 metabolism research and related clinical assays, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your development workflow.