[KD Validated] Anti-PCSK9 (Proprotein Convertase 9) Monoclonal Antibody for WB, ELISA - Q8NBP7

Core Product Specifications and Parameters

Biological Background: PCSK9 Function and Localization

• Crucial regulator of plasma cholesterol homeostasis; binds to LDL receptor family members (LDLR, VLDLR, LRP1/APOER, LRP8/APOER2) and promotes their degradation in intracellular acidic compartments, preventing LDLR recycling. Related references: PMID:18039658 PMID:17461796 PMID:18197702 PMID:18799458
• Acts via a non-proteolytic, clathrin-mediated pathway; induces ubiquitination of LDLR leading to lysosomal degradation.
• Involved in disposal of non-acetylated BACE1 intermediates in the early secretory pathway. Related references: PMID:18660751
• Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway; regulates ENaC-mediated Na⁺ absorption and neuronal apoptosis through LRP8/APOER2 modulation.
• Subcellular localization includes cytoplasm, secreted, endosome, lysosome, cell surface, endoplasmic reticulum, and Golgi apparatus.
• Post-translational modifications: glycoprotein, disulfide bonds, phosphoprotein, sulfation, autocatalytic cleavage from zymogen.
• Tissue specificity: expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and Schwann cells.

Experimental Guidance and Technical Tips

• For western blot, expect a major band near 74 kDa (full-length precursor); autocatalytic cleavage may produce smaller fragments. Validate under reducing/non-reducing conditions in your sample system.
• The immunogen corresponds to the C-terminal region (aa 593–692); ensure epitope accessibility in denaturing vs. native conditions.
• For ELISA, consider testing antibody pair compatibility; steric hindrance with other clones may affect sensitivity.
• Cross-reactivity confirmed for human; verify for other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable access to validated IVD raw materials, including monoclonal antibodies, recombinant proteins, and bulk ancillary reagents. For PCSK9-related research in cholesterol metabolism and cardiovascular disease, CamelBio offers a comprehensive portfolio of validated antibody pairs, optimized clones, and rare target sourcing services, streamlining your development from concept to clinic.