[KD Validated] Anti-NF2/Merlin Rabbit Monoclonal Antibody for WB, IHC-P and ELISA - P35240

Core Product Specifications and Parameters

Biological Background: NF2/Merlin Function and Localization

• NF2/Merlin (Neurofibromin-2, also known as Schwannomin) is a tumor suppressor protein belonging to the ezrin-radixin-moesin (ERM) family.
• It functions as a regulator of the Hippo signaling pathway, restricting proliferation and promoting apoptosis, thereby suppressing tumorigenesis.
• Merlin localizes to the plasma membrane, particularly at filopodia and ruffles, as well as in the cytoplasm, nucleus, and cytoskeleton, indicating dynamic intracellular roles.
• It interacts with and inhibits the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex, contributing to its tumor suppressor activity.
• Merlin may inhibit PI3 kinase signaling by binding to AGAP2, affecting cell survival and proliferation pathways.
• Post-translational modifications such as phosphorylation regulate its conformation and function, and it is subject to ubiquitination.
• Multiple isoforms exist due to alternative splicing, with isoform 1 and 3 being most widely expressed in adult tissues.

Experimental Guidance and Technical Tips

• This recombinant rabbit monoclonal antibody was generated against amino acids 347-595 of human NF2/Merlin, a region that may be critical for its functional interactions. For Western blot, a band at approximately 70 kDa is expected; consider using appropriate loading controls and confirm specificity with knockdown or knockout samples.
• For immunohistochemistry (IHC-P), antigen retrieval conditions and antibody dilution should be optimized for each tissue type; include isotype controls and validate in known positive and negative tissues.
• ELISA applications can be used for quantitative detection; ensure the coating antigen is compatible with the immunogen region and validate cross-reactivity with mouse and rat orthologs experimentally.
• Since the antibody cross-reacts with mouse and rat, it is suitable for comparative studies in these species; however, verify performance in the relevant model system.

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CamelBio connects diagnostic and research communities with high-quality IVD raw materials. We supply validated antibodies like this anti-NF2/Merlin monoclonal antibody to support your oncology and signaling studies, offering one-stop access to antibody pairs, ancillary reagents, and bulk quantities to streamline your assay development from concept to clinic.