KD Validated Anti-NF2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P35240

Core Product Specifications and Parameters

Biological Background: NF2 Function and Localization

• NF2 (Neurofibromin 2) encodes merlin (moesin-ezrin-radixin-like protein), also known as schwannomin, a member of the ERM protein family.
• Functions as a tumor suppressor by regulating the Hippo/SWH signaling pathway, synergizing with WWC1 to induce LATS1/LATS2 phosphorylation, thereby restricting proliferation and promoting apoptosis.
• Acts as a membrane-cytoskeleton linker, stabilizing the membrane and localizing to cell projections (filopodia, ruffles), cytoplasm (perinuclear region, granules), and nucleus.
• Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.
• May inhibit PI3 kinase by binding to AGAP2.
• Post-translationally modified by phosphorylation and ubiquitination (Ubl conjugation).
• Widely expressed; multiple isoforms (1-10) with isoform 1 and 3 predominant; implicated in deafness and disease variants, notably associated with neurofibromatosis type 2.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 477–576 (C-terminal region) of human NF2; consider using this peptide for blocking or competitive assays to verify specificity.
• Given the KD validation, this antibody is suitable for confirming target knockdown in Western blot experiments.
• For IF/ICC, permeabilization and fixation conditions should be carefully optimized for membrane/cytoskeletal targets to ensure accurate localization.
• The predicted molecular weight is approximately 70 kDa; use appropriate gel percentages and loading controls to resolve the band clearly.
• Always validate the antibody in your specific sample matrix and experimental conditions, as performance can vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to a broad portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-NF2 product, and bulk ancillary reagents. Our one-stop platform supports researchers focusing on the Hippo pathway and tumor-suppressor mechanisms by sourcing rare-target reagents and offering integrated technical and procurement solutions. Whether you require established catalog products or custom-sourced biomaterials, CamelBio bridges the gap from concept to clinic.