KD Validated Anti-NDUFA13/GRIM19 Polyclonal Antibody for WB, ELISA - Q9P0J0

Core Product Specifications and Parameters

Biological Background: NDUFA13/GRIM19 Function and Localization

• NDUFA13/GRIM19 is an accessory subunit of mitochondrial membrane respiratory chain Complex I that is not directly involved in catalysis, but is essential for electron transfer from NADH to ubiquinone. Related references: PMID:27626371
• It mediates interferon/all-trans-retinoic acid (IFN/RA)-induced apoptosis, and its apoptotic activity is inhibited by interaction with viral IRF1.
• The protein prevents transactivation of STAT3 target genes and plays a role in CARD15-mediated innate mucosal responses, regulating intestinal epithelial cell responses to microbes. Related references: PMID:15753091
• Subcellular localization includes the mitochondrial inner membrane and the nucleus.
• Widely expressed, with highest levels in heart, skeletal muscle, liver, kidney, and placenta; down-regulated in intestinal mucosa areas affected by Crohn disease and ulcerative colitis.
• Post-translational modifications include acetylation; the gene undergoes alternative splicing.
• Key associated terms: primary mitochondrial disease, host-virus interaction, and apoptosis.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-144) of NDUFA13/GRIM19, a region containing known functional domains – this design may favor recognition of native conformations in Western blot (WB) and ELISA.
• In WB, expect a band at approximately 17 kDa (predicted molecular weight 16.7 kDa plus potential modifications); validate specificity using knockdown (KD) or knockout models when available.
• For ELISA, consider pairing this polyclonal antibody with a matched monoclonal capture or detection antibody; perform cross-reactivity and matrix effect assessments in the relevant sample system.
• Note that the antibody reportedly reacts with human, mouse, and rat samples; verify species-specific performance in your assay conditions.
• Optimization of dilution factors and incubation conditions is recommended based on the specific experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, technical services, and consulting—from concept to clinic. This NDUFA13/GRIM19 antibody supports research in mitochondrial biology, apoptosis, and inflammatory diseases. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare-target raw materials to streamline your diagnostic development.