[KD Validated] Anti-MRPS18C Rabbit Polyclonal Antibody for WB, ELISA - Q9Y3D5

Core Product Specifications and Parameters

Biological Background: MRPS18C Function and Localization

• The protein is officially designated as small ribosomal subunit protein bS18m, also known as MRPS18C, 28S ribosomal protein S18-1 mitochondrial, and MRP-S18-1.
• It is a component of the mitochondrial small ribosomal subunit (28S), where it is expected to contribute to the structural integrity of the ribosome and mitochondrial protein synthesis.
• Subcellular localization is mitochondrial, directed by an N-terminal transit peptide that is cleaved after import.
• MRPS18C belongs to the ribonucleoprotein class, associating with mitochondrial ribosomal RNA to form functional ribosomal particles.
• The protein has been identified in proteomics screens and is included in the reference proteome, underscoring its ubiquitous expression.
• Its calculated molecular weight is approximately 15.85 kDa, consistent with the observed band at 15 kDa, and no post-translational modifications are currently annotated.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–100, the N-terminal region. Consider peptide competition assays or deletion constructs to confirm epitope specificity.
• For Western blot, use mitochondrial-enriched fractions or whole-cell lysates from human cell lines; expect a clean band near 15 kDa under reducing conditions.
• ELISA setups can employ coated recombinant MRPS18C or the immunogenic peptide; titrate the antibody to determine the optimal working dilution.
• As a polyclonal antibody, it may detect multiple epitopes; validate specificity using siRNA-mediated knockdown (KD) or CRISPR knockout controls.
• Cross-reactivity is listed as human; if testing other species, align the immunogen sequence with orthologous proteins to predict reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges the gap between research and diagnostics with a comprehensive portfolio of IVD raw materials, including this anti-MRPS18C antibody for mitochondrial translation studies. Our sourcing capabilities extend to validated antibody pairs, optimized monoclonal/polyclonal preparations, and rare-target reagents, allowing you to assemble complete assay systems with minimal development overhead. Whether you need a single critical antibody or a fully matched raw-material set, CamelBio streamlines procurement so you can focus on assay development.