KD Validated Anti-MRPL53 Rabbit Polyclonal Antibody for WB, ELISA - Q96EL3

Core Product Specifications and Parameters

Biological Background: MRPL53 Function and Localization

• MRPL53 encodes the mitochondrial large ribosomal subunit protein mL53, also known as 39S ribosomal protein L53, mitochondrial (L53mt or MRP-L53), with a predicted molecular weight of approximately 12 kDa.
• mL53 is a component of the mitochondrial ribosome (mitoribosome), specifically the large subunit (39S), which mediates mitochondrial protein synthesis.
• The protein is encoded by the nuclear genome, synthesized in the cytosol, and imported into mitochondria via an N-terminal transit peptide.
• Subcellular localization studies confirm that mL53 resides in the mitochondrion, consistent with its role in organellar translation.
• As a ribosomal protein, mL53 is part of a ribonucleoprotein complex that facilitates mRNA translation for the 13 mitochondrially encoded proteins of the oxidative phosphorylation system.
• Keywords associated with mL53 include "Mitochondrion", "Ribonucleoprotein", and "Transit peptide", reflecting its organelle-specific translation function and import mechanism.

Experimental Guidance and Technical Tips

• For Western blot (WB) analysis, consider using mitochondrial-enriched fractions or whole cell lysates from human cell lines. Expect a band at approximately 12 kDa.
• Specificity can be validated using siRNA- or shRNA-mediated MRPL53 knockdown cell lines as negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and research laboratories, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized raw-material procurement. This anti-MRPL53 antibody assists in mitochondrial research and diagnostic assay development, and our team can support your supply chain with scale-up, custom formulations, and rare-target sourcing solutions.